Ing. DI (FH) Dr. Harald Kühnel, MSc Academic Staffharald.email@example.com T: +43 1 606 68 77-3603 F: +43 1 606 68 77-3609 Room: MUT.03 Muthgasse 11/2 1190 WienPersonal WebspaceLectures 2022/23Applied Life Sciences> Implementation of Analytical Methods UE Biotechnological Quality Management moreImplementation of Analytical Methods UELector: Horia-Paul Bunduc, BSc, Ing. DI (FH) Dr. Harald Kühnel, MSc, Laurentius Orsolic, BSc1.5SWS2ECTSLecture contentsImplementing an analytical method to practice the validation of analytical methods in depthAssessment methodsinteractive participation Assessment of practical work and authoring of all validation documentsTeaching methodsLectured practicalLanguageGerman> Molecular Biology Laboratory Practical LB Bioprocess Technology moreMolecular Biology Laboratory Practical LBLector: Atefeh Ebrahimian, Ing. DI (FH) Dr. Harald Kühnel, MSc, Laurentius Orsolic, BSc1SWS2ECTSLecture contentsIntroduction to eukaryotic (mammalian) cell culture Basic Methods: Thawing, Splitting, Seeding, Harvesting, Freezing, Transfection methods: Co-precipitation and lipofection Influence of e.g. different plasmid backbones or different host cell lines on product Analysis: Fluorometric, cytotoxicity, protein content (specific vs. total)Assessment methodsContinuous assessment of course work/protocolTeaching methodsLecture, experimentation, discussionLanguageGerman-EnglishPublications The publications of Harald Kühnel written at FH Campus Wien can be found in our publication database, others in the personal web space.