Personendetail

Ing. DI (FH) Dr. Harald Kühnel, MSc

Academic Staff


T: +43 1 606 68 77-3603
F: +43 1 606 68 77-3609

Room: MUT.03
Muthgasse 11/2
1190 Wien


Lectures 2019/20

Applied Life Sciences

> Animal Cell Technology VO
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Animal Cell Technology VO

Lector: Ing. DI (FH) Dr. Harald Kühnel, MSc, Dipl.-Ing. Dr. Sylvia Weilner

1 SWS
2 ECTS

Lecture contents

Kühnel

1. Isolation of cells
2. Hayflicklimit, telomeres and telomerase
3. Specialised cells
4. Immortalisation of cell lines
5. Tissue engineering, Organkultur

Weilner
1. Cell culture laboratories, steril technique and cryoconservation
2. Cultivation methods, cell number, media and components
3. Cell line characterisation
4. Application of animal cell lines and development of recombinant cell lines

Assessment methods

written

Teaching methods

Classroom lecture

Language

German-English

> Bachelor Thesis - Supervision - BVT VO+UE
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Bachelor Thesis - Supervision - BVT VO+UE

Lector: Hon.-Prof. Univ.-Doz. DI Dr. Rudolf Friedrich Bliem, Ing. DI (FH) Dr. Harald Kühnel, MSc, FH-Prof. DI Dr. Michael Maurer

2 SWS
2 ECTS

Lecture contents

See german description

Assessment methods

see german description

Teaching methods

See german description

> Bioanalytics VO
Bioengineeringmore

Bioanalytics VO

Lector: Ing. DI (FH) Dr. Harald Kühnel, MSc

2 SWS
3 ECTS

Lecture contents

Theoretical part:
Immunological techniques like ELISA, SPR, Western-blot, Protein-arrays, IHC, …
Techniques of cell biology like fluorescence microscopy, FACS, counting of cells, …
Fundamental biochemical techniques like determining the concentration of protein solutions, …
And instrumental analysis-techniques like electrophoresis, capillary-electrophoresis, FPLC, HPLC, Proteome-analysis, … .
DNA-analysis: qPCR and digital PCR

Part Schlager:
Extraction and analysis of Proteins by FPLC
DNA-analysis: staining of DNA, sequencing, electrophoresis, ....

Practical part:
Students will solve bio-analytical problems by using recent literature (pubmed, …) and condense their findings in a 20-minute lecture including literature citrates.

Possible bio analytic questions:
What are exosomes, find out how exosomes can be extracted out of ascites. Describe at least two possible extraction methods. Finally, you should prove that you did a successful extraction and yielded an as clean as possible exosomal fraction without contamination with lipoproteins.

Assessment methods

Exam at the end of the lecture active participation, speeches

Teaching methods

lecture and group work

> Biochemistry (practical Course) LB
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Biochemistry (practical Course) LB

Lector: Barbara Eckmair, Bakk. techn., Miriam Hornung, Ing. DI (FH) Dr. Harald Kühnel, MSc, Thomas Schmidt, DI Dr. Gerhard Stadlmayr

2.5 SWS
3 ECTS

Lecture contents

Chemical Calculations, NiNTA Chromatopraphy, Dialysis, Enzyme activity assays, Enzyme kinetic assays, SEC and HPLC, Protein quantification (Bradford, OD280), SDS-PAGE, Western Blot, ELISA

Assessment methods

In-class participation and written protocols

Teaching methods

Practical training accompanied by discussion and reflection rounds

Language

German

> Cell Biology VO
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Cell Biology VO

Lector: Ing. DI (FH) Dr. Harald Kühnel, MSc, FH- Prof.in Mag.a Dr.in Marianne Raith

2 SWS
4 ECTS

Lecture contents

The cell (Organization, cell compartments, Cytoskeleton); cell membrane (structure, properties, lipid and protein components, transport mechanisms); cell organelles.; cell nucleus, storage and information, gene therapy; proteins and their manifold functions; Bioenergetics; cell Division; Tissues; Cellular components of the immune system, genetic and epigenetic mechanisms to control cellular behavior

Assessment methods

Written Exam

Teaching methods

Powerpoint slides
Discussion rounds following each chapter, to reflect on the presented information

Language

German-English

> Facility Design, GMP-Project, Bachelor Thesis SE
Bioengineeringmore

Facility Design, GMP-Project, Bachelor Thesis SE

Lector: Hon.-Prof. Univ.-Doz. DI Dr. Rudolf Friedrich Bliem, Ing. DI (FH) Dr. Harald Kühnel, MSc, FH-Prof. DI Dr. Michael Maurer

5 SWS
10 ECTS

Lecture contents

see german description

Assessment methods

See german description

Teaching methods

Seminar, homework

Language

German

> Introduction to the biochemical Exercises VO+UE
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Introduction to the biochemical Exercises VO+UE

Lector: Ing. DI (FH) Dr. Harald Kühnel, MSc

0.5 SWS
1 ECTS

Lecture contents

Methods for Proteins:
- Total protein concentration
- Immunochemistry (ELISA)
- Electrophoresis
- Chromatography
- Mass spectrometry

Enzyme kinetics
- Km-Value
- Reaction rates

Nucleic Acids

Assessment methods

Homework, In-class contribution, Final Exam

Teaching methods

Lecture with practical examples, which are done in the form of short projects during lectures or as part of homework

> Molecular Genetics (practical Course) - Project Pr...
Bioengineeringmore

Molecular Genetics (practical Course) - Project Preparation SE

Lector: Ing. DI (FH) Dr. Harald Kühnel, MSc, Dr. Christian Leitner

1 SWS
1 ECTS

Lecture contents

Goal:
Establish a plan to produce lactic acid in yeast. Develop a concept including all relevant points and present it to your client.

Goal of this seminar is to clone the L-lactat dehydrogenase gene (LDH, EC 1.1.1.27) into a yeast strain. This will lead to a transgenic organism capable of producing lactic acid.
Plan all necessary steps to produce this strain:
Which yeast will you use? From where will you get it?
LDH: From which organism, where/how will you get the gene?
Vector: Which essential part must the vector contain? Where will you get it, buy, construct….?
How can you clone the LDH gene into the vector? REN, cutting sites, primer….
Which transformation method will you use?
How will you screen for positive transformants? Isolation, master cell bank…
How will you prove the correct gene transfer and the production of lactic acid?
The concept can/should be individual and in theory functional. There is no silver bullet (yeast strain, LDH gene, cloning, transfer, vector, detection…). Lively discussion between colleges is encouraged. Identical or recycled concepts from previous year will not be tolerated!
At the end the concept will be presented to your colleges in the frame of a short presentation.
Length of the written presentation: 2 Pages.

Assessment methods

Graded will be the writen concept and the presentation.

Teaching methods

Independent preparation of the concept and the presentation, training and time for Q&A during the lectures

Language

German

> Molecular Genetics (practical Course) LB
Bioengineeringmore

Molecular Genetics (practical Course) LB

Lector: Ing. DI (FH) Dr. Harald Kühnel, MSc, Maria Schrammel, BSc, Dr. Matthias Steiger

2 SWS
3 ECTS

Lecture contents

Construction of an expression vector to produce l-lactic acid in Saccharomyces cerevisiae
1.) Isolation of the l-lactat-dehydrogenase gen form Lactobacillus plantarum
2.) Construction and amplification of the expression vector in E. coli
3.) Transferring the expression vector in the target organism S. cerevisiae and measurement of the LDH activity

Assessment methods

Writing a scientific lab protocol
Recommended language: German or English
Rated will be the protocol and the practical work in the lab.

Teaching methods

After a theoretical introduction at the beginning of each lab day the students will conduct the experiments by themselves with help from the protocols.

Language

German

> Molecular Genetics and Straindevelopment VO
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Molecular Genetics and Straindevelopment VO

Lector: FH-Prof.in Mag.a Dr.in Alexandra Graf, Ing. DI (FH) Dr. Harald Kühnel, MSc

2 SWS
4 ECTS

Lecture contents

Knowledge on basics of molecular genetics:

What are living organisms made of - in a biochemical sense - what molecules - which compartments and structures?
How are these molecules, compartments and structures passed on to offspring?
How do living organisms function in a chemical and physical sense?
How is this live "steered"?
How identical are offspring to their parents, or how different?
How is genetic and biological relationship explained on the molecular level?

Knowledge on basics recombinant DNA technology:

Enzymatic modification of DNA
Amplification of DNA - Polymerase Chain Reaction (PCR)
Gene expression and cloning
Transfer of DNA into host cells
Isolation of genomic and plasmid DNA
Bacteriophages - biology and application
Qualitative and quantitative analytical methods
Expression systems/hosts/vectors
Genomics and Proteomics

Assessment methods

written exam

Teaching methods

Lecture

Language

German

> Practical Course: Fermentation Technology LB
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Practical Course: Fermentation Technology LB

Lector: Ing. Michael Geissler, MSc., Ing. DI (FH) Dr. Harald Kühnel, MSc, Dr. Christian Leitner, Dr. Nico Lingg, FH-Prof. DI Dr. Michael Maurer, Maria Schrammel, BSc

3.5 SWS
5 ECTS

Lecture contents

The course consists of two parts:
1.Brewing technology
2.Characterization of a recombinant yeast strain
In the course of the brewing technology, beer, liquor and vinegar are produced. This includes the process design, QA, filling and labeling.
In the second part, a yeast strain producing lactic acid (outcome of the molecular biological lab) will be characterized in terms of productivity in comparison to the wild type and a mutant strain (PDC neg.)

Assessment methods

Written reports

Teaching methods

Laboratory

Language

German