Details

Lecture contents

Knowledge on basics of molecular genetics:

What are living organisms made of - in a biochemical sense - what molecules - which compartments and structures? How are these molecules, compartments and structures passed on to offspring?
How do living organisms function in a chemical and physical sense? How is this live "steered"?
How identical are offspring to their parents, or how different?
How is genetic and biological relationship explained on the molecular level?

Topics in the Basics part:
- Historical perspective - Genomics, Transcriptomics, Proteomics
- Differences between Eukaryota, Porkaryota, Archaea, and Viruses
- Replication - Transcription - Translation
- Mutations and Recombination

Methods included in this part:
- Sequencing technologies

Assessment methods

Moodle exam

Teaching methods

Lecture

Language

German

Lecture contents

Construction of an expression vector to produce l-lactic acid in Saccharomyces cerevisiae
1.) Isolation of the l-lactat-dehydrogenase gen form Lactobacillus plantarum
2.) Construction and amplification of the expression vector in E. coli
3.) Transferring the expression vector in the target organism S. cerevisiae and measurement of the LDH activity

Assessment methods

Writing a scientific lab protocol
Recommended language: German or English
Rated will be the protocol and the practical work in the lab.

Teaching methods

After a theoretical introduction at the beginning of each lab day the students will conduct the experiments by themselves with help from the protocols.

Language

German

Lecture contents

Goal:
Establish a plan to produce lactic acid in yeast. Develop a concept including all relevant points and present it to your client.

Goal of this seminar is to clone the L-lactat dehydrogenase gene (LDH, EC 1.1.1.27) into a yeast strain. This will lead to a transgenic organism capable of producing lactic acid.
Plan all necessary steps to produce this strain:
Which yeast will you use? From where will you get it?
LDH: From which organism, where/how will you get the gene?
Vector: Which essential part must the vector contain? Where will you get it, buy, construct….?
How can you clone the LDH gene into the vector? REN, cutting sites, primer….
Which transformation method will you use?
How will you screen for positive transformants? Isolation, master cell bank…
How will you prove the correct gene transfer and the production of lactic acid?
The concept can/should be individual and in theory functional. There is no silver bullet (yeast strain, LDH gene, cloning, transfer, vector, detection…). Lively discussion between colleges is encouraged. Identical or recycled concepts from previous year will not be tolerated!
At the end the concept will be presented to your colleges in the frame of a short presentation.
Length of the written presentation: 2 Pages.

Assessment methods

Graded will be the writen concept and the presentation.

Teaching methods

Independent preparation of the concept and the presentation, training and time for Q&A during the lectures

Language

German

Lecture contents

The course consists of two parts:
1. Brewing technology
2. Characterization of a recombinant yeast strain
In the course of the brewing technology, beer, liquor and vinegar are produced. This includes the process design, QA, filling and labeling.
In the second part, a yeast strain producing lactic acid (outcome of the molecular biological lab) will be characterized in terms of productivity in comparison to the wild type and a mutant strain (PDC neg.)

Assessment methods

Written reports

Teaching methods

Laboratory

Language

German

Lecture contents

Kühnel

1. Isolation of cells
2. Hayflicklimit, telomeres and telomerase
3. Specialised cells
4. Immortalisation of cell lines
5. Tissue engineering, Organkultur

Weilner
1. Cell culture laboratories, steril technique and cryoconservation
2. Cultivation methods, cell number, media and components
3. Cell line characterisation
4. Application of animal cell lines and development of recombinant cell lines

Assessment methods

written

Teaching methods

Classroom lecture

Language

German-English

Lecture contents

Implementing an analytical method to practice the validation of analytical methods in depth

Assessment methods

interactive participation

Assessment of practical work and authoring of all validation documents

Teaching methods

Lectured practical

Language

German

Lecture contents

Methods for Proteins:
- Total protein concentration
- Immunochemistry (ELISA)
- Electrophoresis
- Chromatography
- Mass spectrometry

Enzyme kinetics
- Km-Value
- Reaction rates

Nucleic Acids

Assessment methods

Homework, In-class contribution, Final Exam

Teaching methods

Lecture with practical examples, which are done in the form of short projects during lectures or as part of homework

Lecture contents

Introduction to eukaryotic (mammalian) cell culture
Basic Methods: Thawing, Splitting, Seeding, Harvesting, Freezing,
Transfection methods: Co-precipitation and lipofection
Influence of e.g. different plasmid backbones or different host cell lines on product
Analysis: Fluorometric, cytotoxicity, protein content (specific vs. total)

Assessment methods

Continuous assessment of course work/protocol

Teaching methods

Lecture, experimentation, discussion

Language

German-English