Ing. DI (FH) Dr. Harald Kühnel, MSc
Academic Staff
harald.kuehnel@fh-campuswien.ac.at
+43 1 606 68 77-3603
+43 1 606 68 77-3609
Room: E.3.04
Favoritenstraße 222
1100 Wien
Lectures 2022/23
Applied Life Sciences
Implementation of Analytical Methods UE
Lector: Horia-Paul Bunduc, BSc, Ing. DI (FH) Dr. Harald Kühnel, MSc, Laurentius Orsolic, BSc
Lecture contents
Implementing an analytical method to practice the validation of analytical methods in depth
Assessment methods
interactive participation
Assessment of practical work and authoring of all validation documents
Teaching methods
Lectured practical
Language
German
Data Analysis Laboratory LB
Lector: FH-Prof.in Mag.a Dr.in Alexandra Graf, Ing. DI (FH) Dr. Harald Kühnel, MSc, Laurentius Orsolic, BSc, Monika Waldherr, MSc
Lecture contents
-) Introduction to the Oxford Nanopore sequencing technology
-) Sequencing of DNA samples using MinION in the wet lab
-) Development of suitable quality control
-) Development of suitable analysis pipeline
-) Interpretation and discussion of results
Assessment methods
Immanent examination, protocol of the results of the practical exercises, final exam
Teaching methods
-) Presentation
-) practical exercises in the wet-lab and in-silico
Language
German
Molecular Biology Laboratory Practical LB
Lector: Atefeh Ebrahimian, MSc, Ing. DI (FH) Dr. Harald Kühnel, MSc, Laurentius Orsolic, BSc
Lecture contents
Introduction to eukaryotic (mammalian) cell culture
Basic Methods: Thawing, Splitting, Seeding, Harvesting, Freezing,
Transfection methods: Co-precipitation and lipofection
Influence of e.g. different plasmid backbones or different host cell lines on product
Analysis: Fluorometric, cytotoxicity, protein content (specific vs. total)
Assessment methods
Continuous assessment of course work/protocol
Teaching methods
Lecture, experimentation, discussion
Language
German-English
Master Thesis Seminar SE
Lector: Ing. DI (FH) Dr. Harald Kühnel, MSc, FH-Prof. DI Dr. Michael Maurer
Lecture contents
In this class each student has to give a scientific presentation of his or her diploma thesis as preparation for the diploma examination
- What has to be considered when presenting scientific work?
- Give and receive feedback.
Assessment methods
Assessment of the given presentation (structure, slide deck, presentation style, timing).
Teaching methods
Presentation
Language
German
Master Thesis Seminar SE
Lector: Ing. DI (FH) Dr. Harald Kühnel, MSc, FH-Prof. DI Dr. Michael Maurer
Lecture contents
In this class each student has to give a scientific presentation of his or her diploma thesis as preparation for the diploma examination
- What has to be considered when presenting scientific work?
- Give and receive feedback.
Assessment methods
Assessment of the given presentation (structure, slide deck, presentation style, timing).
Teaching methods
Presentation
Language
German