FH- Prof. Mag. Dr. Marianne Raith Academic Staffmarianne.raith@fh-campuswien.ac.at T: +43 1 606 68 77-3541 F: +43 1 606 68 77-3509 Room: OG.F.04 Helmut Qualtinger Gasse 2 1030 WienLectures 2021/22Applied Life Sciences> Cell Culture VO Molecular Biotechnology moreCell Culture VOLector: FH- Prof.in Mag.a Dr.in Marianne Raith1SWS1ECTSLecture contents1) Basics of cell- und tissue culture (legal basis, safety levels, requirements and equipment, sterile techniques, contaminations and how to avoid them) 2) The cell and its environment (culture vessels and their treatment, culture conditions) 3) Routine methods for basic handling of cultured cells (medium exchange, subculturing, determination of growth parameters, freezing, thawing and shipment of cells) 4) Cell lines versus primary cells (isolation of primary cells, establishment and characterization of cell lines) 5) Cells as factories (hybridomatechnique for the production of monoclonal antibodies, production of recombinant proteins, transfection, mass cell culture, 3D cell cultures) 6) Methods in cell culture 7) Stemcells (Basics) 8) Plant cell cultures (Basics)Assessment methodsRegular Moodle quizzes Final examTeaching methodsLecture (PowerPoint presentation and lecture videos) Quizzes for self-examinationLanguageGerman> Cell Culture Laboratory LAB Molecular Biotechnology moreCell Culture Laboratory LABLector: Kay Holleis, BSc MSc, Andrea Krames, BSc MSc, FH- Prof.in Mag.a Dr.in Marianne Raith3SWS3ECTSLecture contentsExperiment 1: Handling of Cell Lines (Splitting, Cryoconservation, Live-Dead-Ratio) Experiment 2: Growth Curve (Evaluation of Doubling-Time and the Influence of Changed Culture conditions) Experiment 3: Cell cycle/Mitosis Experiment 4: Cytoskeleton/Transfection Experiment 5: Problem-Based LearningAssessment methodsOpening exam (Moodle) Active participation during the whole course (technical performance and participation) closing discussion (similar to oral exam) and presentation of the problem-based question (in groups) every student has to write a protocol describing the experimental part of the course (deadline 2 weeks after the end of the laboratory course, see Moodle for further details)Teaching methodsPreliminary discussion of the theoretical background of the experiments and practical implementation of the experiments. Problem-based learning.LanguageGerman> Scientific Method: Immunology SE Molecular Biotechnology moreScientific Method: Immunology SELector: FH- Prof.in Mag.a Dr.in Marianne Raith, FH-Prof.in Univ.Doz.in Dr.in Ines Swoboda1SWS1ECTSLecture contentsIn this course the students learn strategies of scientific research. In a small group they develop a strategy for a given topic (in Immunology), by designing experiments and work packages which they evaluate according to different criteria like costs, effort, efficiency. Finally each group presents its research project.Assessment methodsFinal exam Presentation and discussion of the developed strategyTeaching methodsSeminarLanguageEnglishPublications The publications of Marianne Raith written at FH Campus Wien can be found in our publication database, others in the personal web space.
> Cell Culture VO Molecular Biotechnology moreCell Culture VOLector: FH- Prof.in Mag.a Dr.in Marianne Raith1SWS1ECTSLecture contents1) Basics of cell- und tissue culture (legal basis, safety levels, requirements and equipment, sterile techniques, contaminations and how to avoid them) 2) The cell and its environment (culture vessels and their treatment, culture conditions) 3) Routine methods for basic handling of cultured cells (medium exchange, subculturing, determination of growth parameters, freezing, thawing and shipment of cells) 4) Cell lines versus primary cells (isolation of primary cells, establishment and characterization of cell lines) 5) Cells as factories (hybridomatechnique for the production of monoclonal antibodies, production of recombinant proteins, transfection, mass cell culture, 3D cell cultures) 6) Methods in cell culture 7) Stemcells (Basics) 8) Plant cell cultures (Basics)Assessment methodsRegular Moodle quizzes Final examTeaching methodsLecture (PowerPoint presentation and lecture videos) Quizzes for self-examinationLanguageGerman
> Cell Culture Laboratory LAB Molecular Biotechnology moreCell Culture Laboratory LABLector: Kay Holleis, BSc MSc, Andrea Krames, BSc MSc, FH- Prof.in Mag.a Dr.in Marianne Raith3SWS3ECTSLecture contentsExperiment 1: Handling of Cell Lines (Splitting, Cryoconservation, Live-Dead-Ratio) Experiment 2: Growth Curve (Evaluation of Doubling-Time and the Influence of Changed Culture conditions) Experiment 3: Cell cycle/Mitosis Experiment 4: Cytoskeleton/Transfection Experiment 5: Problem-Based LearningAssessment methodsOpening exam (Moodle) Active participation during the whole course (technical performance and participation) closing discussion (similar to oral exam) and presentation of the problem-based question (in groups) every student has to write a protocol describing the experimental part of the course (deadline 2 weeks after the end of the laboratory course, see Moodle for further details)Teaching methodsPreliminary discussion of the theoretical background of the experiments and practical implementation of the experiments. Problem-based learning.LanguageGerman
> Scientific Method: Immunology SE Molecular Biotechnology moreScientific Method: Immunology SELector: FH- Prof.in Mag.a Dr.in Marianne Raith, FH-Prof.in Univ.Doz.in Dr.in Ines Swoboda1SWS1ECTSLecture contentsIn this course the students learn strategies of scientific research. In a small group they develop a strategy for a given topic (in Immunology), by designing experiments and work packages which they evaluate according to different criteria like costs, effort, efficiency. Finally each group presents its research project.Assessment methodsFinal exam Presentation and discussion of the developed strategyTeaching methodsSeminarLanguageEnglish