Details

FH- Prof. Mag. Dr. Marianne Raith

Academic Staff


T: +43 1 606 68 77-3541
F: +43 1 606 68 77-3509

Room: OG.F.04
Helmut Qualtinger Gasse 2
1030 Wien


Lectures 2021/22

Applied Life Sciences

> Cell Culture VO
Molecular Biotechnology more

Cell Culture VO

Lector: FH- Prof.in Mag.a Dr.in Marianne Raith

1SWS
1ECTS

Lecture contents

1) Basics of cell- und tissue culture (legal basis, safety levels, requirements and equipment, sterile techniques, contaminations and how to avoid them)
2) The cell and its environment (culture vessels and their treatment, culture conditions)
3) Routine methods for basic handling of cultured cells (medium exchange, subculturing, determination of growth parameters, freezing, thawing and shipment of cells)
4) Cell lines versus primary cells (isolation of primary cells, establishment and characterization of cell lines)
5) Cells as factories (hybridomatechnique for the production of monoclonal antibodies, production of recombinant proteins, transfection, mass cell culture, 3D cell cultures)
6) Methods in cell culture
7) Stemcells (Basics)
8) Plant cell cultures (Basics)

Assessment methods

Regular Moodle quizzes
Final exam

Teaching methods

Lecture (PowerPoint presentation and lecture videos)
Quizzes for self-examination

Language

German

> Cell Culture Laboratory LAB
Molecular Biotechnology more

Cell Culture Laboratory LAB

Lector: Kay Holleis, BSc MSc, Andrea Krames, BSc MSc, FH- Prof.in Mag.a Dr.in Marianne Raith

3SWS
3ECTS

Lecture contents

Experiment 1: Handling of Cell Lines (Splitting, Cryoconservation, Live-Dead-Ratio)
Experiment 2: Growth Curve (Evaluation of Doubling-Time and the Influence of Changed Culture conditions)
Experiment 3: Cell cycle/Mitosis
Experiment 4: Cytoskeleton/Transfection
Experiment 5: Problem-Based Learning

Assessment methods

Opening exam (Moodle)
Active participation during the whole course (technical performance and participation)
closing discussion (similar to oral exam) and presentation of the problem-based question (in groups)
every student has to write a protocol describing the experimental part of the course (deadline 2 weeks after the end of the laboratory course, see Moodle for further details)

Teaching methods

Preliminary discussion of the theoretical background of the experiments and practical implementation of the experiments. Problem-based learning.

Language

German

> Scientific Method: Immunology SE
Molecular Biotechnology more

Scientific Method: Immunology SE

Lector: FH- Prof.in Mag.a Dr.in Marianne Raith, FH-Prof.in Univ.Doz.in Dr.in Ines Swoboda

1SWS
1ECTS

Lecture contents

In this course the students learn strategies of scientific research. In a small group they develop a strategy for a given topic (in Immunology), by designing experiments and work packages which they evaluate according to different criteria like costs, effort, efficiency. Finally each group presents its research project.

Assessment methods

Final exam
Presentation and discussion of the developed strategy

Teaching methods

Seminar

Language

English

Publications

The publications of Marianne Raith written at FH Campus Wien can be found in our publication database, others in the personal web space.