FH- Prof.in Mag.a Dr.in Marianne Raith Academic Staff marianne.raith@fh-campuswien.ac.at T: +43 1 606 68 77-3541 F: +43 1 606 68 77-3509Room: OG.F.04 Helmut Qualtinger Gasse 2 1030 Wien Lectures 2019/20 Applied Life Sciences > Cell Biology VOBioengineeringmore Cell Biology VO Lector: Ing. DI (FH) Dr. Harald Kühnel, MSc, FH- Prof.in Mag.a Dr.in Marianne Raith 2 SWS 4 ECTS Lecture contents The cell (Organization, cell compartments, Cytoskeleton); cell membrane (structure, properties, lipid and protein components, transport mechanisms); cell organelles.; cell nucleus, storage and information, gene therapy; proteins and their manifold functions; Bioenergetics; cell Division; Tissues; Cellular components of the immune system, genetic and epigenetic mechanisms to control cellular behavior Assessment methods Written Exam Teaching methods Powerpoint slidesDiscussion rounds following each chapter, to reflect on the presented information Language German-English > Cell Culture Laboratory LABMolecular Biotechnologymore Cell Culture Laboratory LAB Lector: Dr. Radostina Bachmaier, FH- Prof.in Mag.a Dr.in Marianne Raith, Andrea Steinbauer, BSc MSc 3 SWS 3 ECTS Lecture contents Experiment 1: Handling of Cell Lines (Splitting, Cryoconservation, Live-Dead-Ratio)Experiment 2: Growth Curve (Evaluation of Doubling-Time and the Influence of Changed Culture conditions)Experiment 3: Cell cycle/MitosisExperiment 4: Cytoskeleton/TransfectionExperiment 5: Problem-Based Learning Assessment methods Opening exam (Moodle)Active participation during the whole course (technical performance and participation)closing discussion (similar to oral exam) and presentation of the problem-based question (in groups) every student has to write a protocol describing the experimental part of the course (deadline 2 weeks after the end of the laboratory course, see Moodle for further details) Teaching methods Preliminary discussion of the theoretical background of the experiments and practical implementation of the experiments. Problem-based learning. Language German > Cell Culture VOMolecular Biotechnologymore Cell Culture VO Lector: FH- Prof.in Mag.a Dr.in Marianne Raith 1 SWS 1 ECTS Lecture contents 1) Basics of cell- und tissue culture (legal basis, safety levels, requirements and equipment, sterile techniques, contaminations and how to avoid them)2) The cell and its environment (culture vessels and their treatment, culture conditions)3) Routine methods for basic handling of cultured cells (medium exchange, subculturing, determination of growth parameters, freezing, thawing and shipment of cells)4) Cell lines versus primary cells (isolation of primary cells, establishment and characterization of cell lines)5) Cells as factories (hybridomatechnique for the production of monoclonal antibodies, production of recombinant proteins, transfection, mass cell culture, 3D cell cultures)6) Methods in cell culture7) Stemcells (Basics)8) Plant cell cultures (Basics) Assessment methods Regular Moodle quizzes (10%)Final exam on Moodle (90%) Teaching methods Lecture (PowerPoint presentation and short demonstration videos) Language German PublicationsThe publications of Marianne Raith written at FH Campus Wien can be found in our publication database, others in the personal web space.
> Cell Biology VOBioengineeringmore Cell Biology VO Lector: Ing. DI (FH) Dr. Harald Kühnel, MSc, FH- Prof.in Mag.a Dr.in Marianne Raith 2 SWS 4 ECTS Lecture contents The cell (Organization, cell compartments, Cytoskeleton); cell membrane (structure, properties, lipid and protein components, transport mechanisms); cell organelles.; cell nucleus, storage and information, gene therapy; proteins and their manifold functions; Bioenergetics; cell Division; Tissues; Cellular components of the immune system, genetic and epigenetic mechanisms to control cellular behavior Assessment methods Written Exam Teaching methods Powerpoint slidesDiscussion rounds following each chapter, to reflect on the presented information Language German-English
> Cell Culture Laboratory LABMolecular Biotechnologymore Cell Culture Laboratory LAB Lector: Dr. Radostina Bachmaier, FH- Prof.in Mag.a Dr.in Marianne Raith, Andrea Steinbauer, BSc MSc 3 SWS 3 ECTS Lecture contents Experiment 1: Handling of Cell Lines (Splitting, Cryoconservation, Live-Dead-Ratio)Experiment 2: Growth Curve (Evaluation of Doubling-Time and the Influence of Changed Culture conditions)Experiment 3: Cell cycle/MitosisExperiment 4: Cytoskeleton/TransfectionExperiment 5: Problem-Based Learning Assessment methods Opening exam (Moodle)Active participation during the whole course (technical performance and participation)closing discussion (similar to oral exam) and presentation of the problem-based question (in groups) every student has to write a protocol describing the experimental part of the course (deadline 2 weeks after the end of the laboratory course, see Moodle for further details) Teaching methods Preliminary discussion of the theoretical background of the experiments and practical implementation of the experiments. Problem-based learning. Language German
> Cell Culture VOMolecular Biotechnologymore Cell Culture VO Lector: FH- Prof.in Mag.a Dr.in Marianne Raith 1 SWS 1 ECTS Lecture contents 1) Basics of cell- und tissue culture (legal basis, safety levels, requirements and equipment, sterile techniques, contaminations and how to avoid them)2) The cell and its environment (culture vessels and their treatment, culture conditions)3) Routine methods for basic handling of cultured cells (medium exchange, subculturing, determination of growth parameters, freezing, thawing and shipment of cells)4) Cell lines versus primary cells (isolation of primary cells, establishment and characterization of cell lines)5) Cells as factories (hybridomatechnique for the production of monoclonal antibodies, production of recombinant proteins, transfection, mass cell culture, 3D cell cultures)6) Methods in cell culture7) Stemcells (Basics)8) Plant cell cultures (Basics) Assessment methods Regular Moodle quizzes (10%)Final exam on Moodle (90%) Teaching methods Lecture (PowerPoint presentation and short demonstration videos) Language German