FH-Prof. Dr. Herbert Wank Academic Staffherbert.wank@fh-campuswien.ac.at T: +43 1 606 68 77-3514 F: +43 1 606 68 77-3509 Room: OG.A.04 Helmut Qualtinger Gasse 2 1030 WienLectures 2021/22Applied Life Sciences> English in Science & Career I ILV Molecular Biotechnology moreEnglish in Science & Career I ILVLector: FH-Prof. Dr. Herbert Wank, FH-Prof. Dr. Paul Watson2SWS2ECTSLecture contentsThe course will deal with all four areas of language skills: reading/writing/talking/listening. The emphasis in the 3rd semester will be on spoken English (presentations (spontaneous and prepared)). Oral presentations will be an important part of the semesters's work. Grammar revision (where appropriate) will be offerred. ACTIVE PARTICIPATION IN THE LESSON IS VERY IMPORTANT AND WILL BE GRADED!Assessment methodsYour command of English, both spoken and written, will be continuously assessed during the semester and will be reflected in the final semester mark. Moreover your active participation in the lessons will also contribute to the overall mark.Teaching methodsSpontaneous and prepared presentations. Brainstormings, Discussions, Debates. ‘Language clinics’. Individual, pair- and group-work. Self-reflection is a major focus of the course.LanguageEnglish> Industry Practical PR Molecular Biotechnology moreIndustry Practical PRLector: FH-Prof. Dr. Herbert Wank, FH-Prof. Dr. Paul Watson0SWS25ECTSLecture contentsThe internship serves as an introduction for the students to work independently. The tasks begin with the search for a suitable internship and internship supervisor. Students learn under the supervision of a professional the relevant professional practice in a biotechnology company/research institute and/or independent scientific work. During the internship the subject-, methodological and social competencies acquired during the study, will be implemented in the desired field of occupational activity and practically consolidated. Another important teaching content is to summarize the internship results in form of an internship report and the documentation of scientific results.Assessment methodsAssessment of practical supervisorTeaching methodsPracticalLanguageGerman-English> Molecular Biology & Genetics I VO Molecular Biotechnology moreMolecular Biology & Genetics I VOLector: FH-Prof. Dr. Herbert Wank2SWS3ECTSLecture contentsStudents learn the basics of genetics and molecular biology with the following topics: • Genetics - Mendel • Classic genetics - gene mapping • genetic defects • Nucleic acids (DNA, RNA) - structure and function • Genome structure, chromatin and nucleosomes • chromosomes • Replication of the DNA • cell cycle • Mitosis - meiosis • Mutations and repair mechanisms • Homologous recombination • Sequence-specific recombination • Transposable elementsAssessment methodsWritten exam on the last day of the course or in coordination with students.Teaching methodsLecture Powerpoint presentations VideosLanguageGerman> Introduction to Molecular Biological Lab Technique… Molecular Biotechnology moreIntroduction to Molecular Biological Lab Techniques LABLector: Kay Holleis, BSc MSc, FH-Prof. Dr. Herbert Wank, FH-Prof. Dr. Paul Watson1SWS1ECTSLecture contentsIn this course the students will get a crash-course in "how to handle an automatic pipet". Then the students perform several restriction digestions in order to map an unknown piece of DNA. At the end of the course, the students determine the concentration of an unknown DNA-Sample spectrophotometrically (including calculation).Assessment methodsEnthusiasm, Protocol in EnglishTeaching methodsIndependent work in the laboratory Introductory remarks from the lector/tutor Independent drawing of a plasmid map Writing of a protocolLanguageGerman> Genetic Engineering LAB Molecular Biotechnology moreGenetic Engineering LABLector: Kay Holleis, BSc MSc, Gregor Sommerkamp, BSc., FH-Prof. Dr. Herbert Wank, ao. Univ.-Prof. Dipl.-Biol. Dr Angela Witte3SWS3ECTSLecture contentsIn this course students learn the basics of cloning. The DNA of an ORF of a phage is amplified using PCR and cloned into the pUC18 vector. Following methods are performed: PCR, restriction digestion, DNA ligation, competent E. coli, plasmid transformation, selection, identification of the clones. Additionally a deletion mutant is analysed by PCR and nucleic acid will be precipitated. The students also write a scientific protocol in "publication-form."Assessment methodsthe grades include: - protocol - lab participation - written testTeaching methodsPractical course with theoretical backgroundLanguageGerman> Molecular Biological & Biophysical Methods SE Molecular Biotechnology moreMolecular Biological & Biophysical Methods SELector: FH-Prof.in Univ.Doz.in Dr.in Ines Swoboda, FH-Prof. Dr. Herbert Wank1.5SWS3ECTSLecture contentsTopics: Detection of Nucleic Acids PCR Western blot Growth of Microorganisms Antibodies and their use in Molecular Biology Centrifugation Protein Purification Southern and Northern blot Primer and Hybridization Proteinexpression Microarray Flurescence in Molecular Biology Sequencing Isolation and Purification of Nucleic Acids from various Organisms Primer and Hybridisation Primer design for cloning of a geneAssessment methodsPreparation, Oral Presentation, Participation in Discussions, written exam at the end of the seminarTeaching methodsSeminar, topic preparation in small groups (4-5), oral presentation in small groups (9-10), 15 minutes presentation, discussion, preparation of a handoutLanguageGerman> Bachelor Thesis I & Scientific Method SE Molecular Biotechnology moreBachelor Thesis I & Scientific Method SELector: FH-Prof. Dr. Herbert Wank0SWS5ECTSLecture contentsbachelor thesis results frominternshipAssessment methodsGrading of the finished bachelor thesisTeaching methodscompleted bachelor thesisLanguageGerman-English> RNA VO Molecular Biotechnology moreRNA VOLector: FH-Prof. Dr. Herbert Wank1SWS2ECTSLecture contentsRNA fundamentals, RNA structure, catalytic RNAs, RNA processing, RNA splicing, RNA editing, riboswitches, RNA applications, non-coding RNAs, RNAi, RNA world, SELEXAssessment methodsFinal exam Written exam at the end of the course.Teaching methodsLectureLanguageEnglish> RNA Analysis Lab VO Molecular Biotechnology moreRNA Analysis Lab VOLector: Kay Holleis, BSc MSc, FH-Prof. Dr. Herbert Wank3SWS3ECTSLecture contentsRNA methods: - Northern blot (glucose/galactose metabolism in yeast, RNA extraction from yeast, denaturing RNA agarose gel, RNA transfer, specific oligonucleotide hybridization, band detection, quantitative PCR) - EMSA (in vitro transcription with T7 RNA polymerase, RNA purification, RNA folding, native polyacrylamide gel electrophoresis, RNA staining using methylene blue, detection of RNP complexes) - RNA stability (temperature dependence, pH dependence, RNAses)Assessment methodsContinuous assessment Continuous assessment - presence, motivation, participation, practical skills (results), written report.Teaching methodsLaboratoryLanguageEnglishPublications The publications of Herbert Wank written at FH Campus Wien can be found in our publication database, others in the personal web space.
> English in Science & Career I ILV Molecular Biotechnology moreEnglish in Science & Career I ILVLector: FH-Prof. Dr. Herbert Wank, FH-Prof. Dr. Paul Watson2SWS2ECTSLecture contentsThe course will deal with all four areas of language skills: reading/writing/talking/listening. The emphasis in the 3rd semester will be on spoken English (presentations (spontaneous and prepared)). Oral presentations will be an important part of the semesters's work. Grammar revision (where appropriate) will be offerred. ACTIVE PARTICIPATION IN THE LESSON IS VERY IMPORTANT AND WILL BE GRADED!Assessment methodsYour command of English, both spoken and written, will be continuously assessed during the semester and will be reflected in the final semester mark. Moreover your active participation in the lessons will also contribute to the overall mark.Teaching methodsSpontaneous and prepared presentations. Brainstormings, Discussions, Debates. ‘Language clinics’. Individual, pair- and group-work. Self-reflection is a major focus of the course.LanguageEnglish
> Industry Practical PR Molecular Biotechnology moreIndustry Practical PRLector: FH-Prof. Dr. Herbert Wank, FH-Prof. Dr. Paul Watson0SWS25ECTSLecture contentsThe internship serves as an introduction for the students to work independently. The tasks begin with the search for a suitable internship and internship supervisor. Students learn under the supervision of a professional the relevant professional practice in a biotechnology company/research institute and/or independent scientific work. During the internship the subject-, methodological and social competencies acquired during the study, will be implemented in the desired field of occupational activity and practically consolidated. Another important teaching content is to summarize the internship results in form of an internship report and the documentation of scientific results.Assessment methodsAssessment of practical supervisorTeaching methodsPracticalLanguageGerman-English
> Molecular Biology & Genetics I VO Molecular Biotechnology moreMolecular Biology & Genetics I VOLector: FH-Prof. Dr. Herbert Wank2SWS3ECTSLecture contentsStudents learn the basics of genetics and molecular biology with the following topics: • Genetics - Mendel • Classic genetics - gene mapping • genetic defects • Nucleic acids (DNA, RNA) - structure and function • Genome structure, chromatin and nucleosomes • chromosomes • Replication of the DNA • cell cycle • Mitosis - meiosis • Mutations and repair mechanisms • Homologous recombination • Sequence-specific recombination • Transposable elementsAssessment methodsWritten exam on the last day of the course or in coordination with students.Teaching methodsLecture Powerpoint presentations VideosLanguageGerman
> Introduction to Molecular Biological Lab Technique… Molecular Biotechnology moreIntroduction to Molecular Biological Lab Techniques LABLector: Kay Holleis, BSc MSc, FH-Prof. Dr. Herbert Wank, FH-Prof. Dr. Paul Watson1SWS1ECTSLecture contentsIn this course the students will get a crash-course in "how to handle an automatic pipet". Then the students perform several restriction digestions in order to map an unknown piece of DNA. At the end of the course, the students determine the concentration of an unknown DNA-Sample spectrophotometrically (including calculation).Assessment methodsEnthusiasm, Protocol in EnglishTeaching methodsIndependent work in the laboratory Introductory remarks from the lector/tutor Independent drawing of a plasmid map Writing of a protocolLanguageGerman
> Genetic Engineering LAB Molecular Biotechnology moreGenetic Engineering LABLector: Kay Holleis, BSc MSc, Gregor Sommerkamp, BSc., FH-Prof. Dr. Herbert Wank, ao. Univ.-Prof. Dipl.-Biol. Dr Angela Witte3SWS3ECTSLecture contentsIn this course students learn the basics of cloning. The DNA of an ORF of a phage is amplified using PCR and cloned into the pUC18 vector. Following methods are performed: PCR, restriction digestion, DNA ligation, competent E. coli, plasmid transformation, selection, identification of the clones. Additionally a deletion mutant is analysed by PCR and nucleic acid will be precipitated. The students also write a scientific protocol in "publication-form."Assessment methodsthe grades include: - protocol - lab participation - written testTeaching methodsPractical course with theoretical backgroundLanguageGerman
> Molecular Biological & Biophysical Methods SE Molecular Biotechnology moreMolecular Biological & Biophysical Methods SELector: FH-Prof.in Univ.Doz.in Dr.in Ines Swoboda, FH-Prof. Dr. Herbert Wank1.5SWS3ECTSLecture contentsTopics: Detection of Nucleic Acids PCR Western blot Growth of Microorganisms Antibodies and their use in Molecular Biology Centrifugation Protein Purification Southern and Northern blot Primer and Hybridization Proteinexpression Microarray Flurescence in Molecular Biology Sequencing Isolation and Purification of Nucleic Acids from various Organisms Primer and Hybridisation Primer design for cloning of a geneAssessment methodsPreparation, Oral Presentation, Participation in Discussions, written exam at the end of the seminarTeaching methodsSeminar, topic preparation in small groups (4-5), oral presentation in small groups (9-10), 15 minutes presentation, discussion, preparation of a handoutLanguageGerman
> Bachelor Thesis I & Scientific Method SE Molecular Biotechnology moreBachelor Thesis I & Scientific Method SELector: FH-Prof. Dr. Herbert Wank0SWS5ECTSLecture contentsbachelor thesis results frominternshipAssessment methodsGrading of the finished bachelor thesisTeaching methodscompleted bachelor thesisLanguageGerman-English
> RNA VO Molecular Biotechnology moreRNA VOLector: FH-Prof. Dr. Herbert Wank1SWS2ECTSLecture contentsRNA fundamentals, RNA structure, catalytic RNAs, RNA processing, RNA splicing, RNA editing, riboswitches, RNA applications, non-coding RNAs, RNAi, RNA world, SELEXAssessment methodsFinal exam Written exam at the end of the course.Teaching methodsLectureLanguageEnglish
> RNA Analysis Lab VO Molecular Biotechnology moreRNA Analysis Lab VOLector: Kay Holleis, BSc MSc, FH-Prof. Dr. Herbert Wank3SWS3ECTSLecture contentsRNA methods: - Northern blot (glucose/galactose metabolism in yeast, RNA extraction from yeast, denaturing RNA agarose gel, RNA transfer, specific oligonucleotide hybridization, band detection, quantitative PCR) - EMSA (in vitro transcription with T7 RNA polymerase, RNA purification, RNA folding, native polyacrylamide gel electrophoresis, RNA staining using methylene blue, detection of RNP complexes) - RNA stability (temperature dependence, pH dependence, RNAses)Assessment methodsContinuous assessment Continuous assessment - presence, motivation, participation, practical skills (results), written report.Teaching methodsLaboratoryLanguageEnglish