FH-Prof. Dr. Herbert Wank Academic Staffherbert.wank@fh-campuswien.ac.at T: +43 1 606 68 77-3514 F: +43 1 606 68 77-3509 Room: OG.A.04 Helmut Qualtinger Gasse 2 1030 WienPersonal WebspaceLectures 2021/22Applied Life Sciences> Methods of DNA analysis VO Molecular Biotechnology moreMethods of DNA analysis VOLector: FH-Prof. Dr. Herbert Wank1SWS2ECTSLecture contentsBiotechnology recombinant DNA - molecular cloning restriction enzymes, enzymes used for molecular cloning plamids - vectors - cloning systems ligation - transformation expression plasmids - recombinant protein expression molecular cloning strategies bacterial strains for cloning or protein expressionAssessment methodswritten ExamTeaching methodsLectureLanguageGerman> Molecular Biology & Genetics II VO Molecular Biotechnology moreMolecular Biology & Genetics II VOLector: FH-Prof. Dr. Herbert Wank2SWS3ECTSLecture contentsExpression of the genome • Mechanism of transcription (DNA -> RNA) • Splicing of RNA • Translation (RNA -> protein) • The genetic code Regulation of gene expression • Transcriptional regulation in prokaryotes • Transcriptional regulation in eukaryotes • Regulatory RNAs Gene regulation in development and evolution Basic methods of molecular biology • nucleic acids • proteins Signal transduction cell death model organismsAssessment methodsWritten exam on the last day of the course or in coordination with students.Teaching methodsLecture Powerpoint presentations VideosLanguageGerman-English> Cell Biology of the Eukaryotes VO Molecular Biotechnology moreCell Biology of the Eukaryotes VOLector: FH-Prof. Dr. Herbert Wank2SWS3ECTSLecture contentsStructure and function of cellular organelles (Nucleus, Mitochondria, Endoplasmatic Reticulum, Golgi, ect.) and substructures (cytoskeleton). Composition, properties and functional relevance of biomembranes. Ion channel and carrier mediated transport of small molecules through membranes. Protein transport into organelles as well as in and out of cells (endocytosis/secretion). The cytoskeleton: composition, regulatory proteins and intracellular transport events. Contact/communication between cells via junctions; the concept of tissues and the extracellular matrix. Complex processes that integrate several features: propagation of action potential along nerve cells; muscle contraction, energy conversion in mitochondriaAssessment methodswritten examTeaching methodsPower point presentationLanguageGerman> English in Science & Career I ILV Molecular Biotechnology moreEnglish in Science & Career I ILVLector: FH-Prof. Dr. Herbert Wank, FH-Prof. Dr. Paul Watson2SWS2ECTSLecture contentsThe course will deal with all four areas of language skills: reading/writing/talking/listening. The emphasis in the 3rd semester will be on spoken English (presentations (spontaneous and prepared)). Oral presentations will be an important part of the semesters's work. Grammar revision (where appropriate) will be offerred. ACTIVE PARTICIPATION IN THE LESSON IS VERY IMPORTANT AND WILL BE GRADED!Assessment methodsYour command of English, both spoken and written, will be continuously assessed during the semester and will be reflected in the final semester mark. Moreover your active participation in the lessons will also contribute to the overall mark.Teaching methodsSpontaneous and prepared presentations. Brainstormings, Discussions, Debates. ‘Language clinics’. Individual, pair- and group-work. Self-reflection is a major focus of the course.LanguageEnglish> Industry Practical PR Molecular Biotechnology moreIndustry Practical PRLector: FH-Prof. Dr. Herbert Wank, FH-Prof. Dr. Paul Watson0SWS25ECTSLecture contentsThe internship serves as an introduction for the students to work independently. The tasks begin with the search for a suitable internship and internship supervisor. Students learn under the supervision of a professional the relevant professional practice in a biotechnology company/research institute and/or independent scientific work. During the internship the subject-, methodological and social competencies acquired during the study, will be implemented in the desired field of occupational activity and practically consolidated. Another important teaching content is to summarize the internship results in form of an internship report and the documentation of scientific results.Assessment methodsAssessment of practical supervisorTeaching methodsPracticalLanguageGerman-English> Molecular Biology & Genetics I VO Molecular Biotechnology moreMolecular Biology & Genetics I VOLector: FH-Prof. Dr. Herbert Wank2SWS3ECTSLecture contentsStudents learn the basics of genetics and molecular biology with the following topics: • Genetics - Mendel • Classic genetics - gene mapping • genetic defects • Nucleic acids (DNA, RNA) - structure and function • Genome structure, chromatin and nucleosomes • chromosomes • Replication of the DNA • cell cycle • Mitosis - meiosis • Mutations and repair mechanisms • Homologous recombination • Sequence-specific recombination • Transposable elementsAssessment methodsWritten exam on the last day of the course or in coordination with students.Teaching methodsLecture Powerpoint presentations VideosLanguageGerman> Introduction to Molecular Biological Lab Technique… Molecular Biotechnology moreIntroduction to Molecular Biological Lab Techniques LABLector: Kay Holleis, BSc MSc, FH-Prof. Dr. Herbert Wank, FH-Prof. Dr. Paul Watson1SWS1ECTSLecture contentsIn this course the students will get a crash-course in "how to handle an automatic pipet". Then the students perform several restriction digestions in order to map an unknown piece of DNA. At the end of the course, the students determine the concentration of an unknown DNA-Sample spectrophotometrically (including calculation).Assessment methodsEnthusiasm, Protocol in EnglishTeaching methodsIndependent work in the laboratory Introductory remarks from the lector/tutor Independent drawing of a plasmid map Writing of a protocolLanguageGerman> Genetic Engineering LAB Molecular Biotechnology moreGenetic Engineering LABLector: Kay Holleis, BSc MSc, Anna Koch, BSc, Andrea Krames, BSc MSc, Gregor Sommerkamp, BSc., FH-Prof. Dr. Herbert Wank, ao. Univ.-Prof. Dipl.-Biol. Dr Angela Witte3SWS3ECTSLecture contentsIn this course students learn the basics of cloning. The DNA of an ORF of a phage is amplified using PCR and cloned into the pUC18 vector. Following methods are performed: PCR, restriction digestion, DNA ligation, competent E. coli, plasmid transformation, selection, identification of the clones. Additionally a deletion mutant is analysed by PCR and nucleic acid will be precipitated. The students also write a scientific protocol in "publication-form."Assessment methodsthe grades include: - protocol - lab participation - written testTeaching methodsPractical course with theoretical backgroundLanguageGerman> Molecular Biological & Biophysical Methods SE Molecular Biotechnology moreMolecular Biological & Biophysical Methods SELector: FH-Prof.in Univ.Doz.in Dr.in Ines Swoboda, FH-Prof. Dr. Herbert Wank1.5SWS3ECTSLecture contentsTopics: Detection of Nucleic Acids PCR Western blot Growth of Microorganisms Antibodies and their use in Molecular Biology Centrifugation Protein Purification Southern and Northern blot Primer and Hybridization Proteinexpression Microarray Flurescence in Molecular Biology Sequencing Isolation and Purification of Nucleic Acids from various Organisms Primer and Hybridisation Primer design for cloning of a geneAssessment methodsPreparation, Oral Presentation, Participation in Discussions, written exam at the end of the seminarTeaching methodsSeminar, topic preparation in small groups (4-5), oral presentation in small groups (9-10), 15 minutes presentation, discussion, preparation of a handoutLanguageGerman> Gene Expression VO Molecular Biotechnology moreGene Expression VOLector: FH-Prof. Dr. Herbert Wank1SWS2ECTSLecture contentsIn this course, the subject areas of the courses "Molecular Biology and Genetics I and II" from the first year of study are partly repeated, deepened and expanded. The individual needs of the students are addressed, i. H. At the beginning of the course, the subject areas to be dealt with are determined together with the students.Assessment methodswritten examTeaching methodsLectureLanguageGerman> Protein Expression & Purification LAB Molecular Biotechnology moreProtein Expression & Purification LABLector: Anna Koch, BSc, Andrea Krames, BSc MSc, Gregor Sommerkamp, BSc., FH-Prof. Dr. Herbert Wank, ao. Univ.-Prof. Dipl.-Biol. Dr Angela Witte3SWS3ECTSLecture contentsThe function of genes is central in this internship where students perform a continuous experiment in a bacterial system. The students get to know methods of protein analysis. The expression of a recombinant protein is first studied in small scale (expression cloning in E. coli). The time course of protein expression is analyzed with the aid of Western blots. After upscaling the culture volume under the previously worked out conditions, the recombinant protein is purified by affinity chromatography (HIS-Tag purification) and finally analyzed, dialyzed and the amount of protein obtained is determined quantitatively. Lab Report: in the form of a scientific publication - abstract, introduction, M&M, results, discussion, literature and citations.Assessment methodsFinal grade: lab report, final examination, motivation, cooperation, practical skills (results)Teaching methodsAlternative Methods> Bachelor Thesis I & Scientific Method SE Molecular Biotechnology moreBachelor Thesis I & Scientific Method SELector: FH-Prof. Dr. Herbert Wank0SWS5ECTSLecture contentsbachelor thesis results frominternshipAssessment methodsGrading of the finished bachelor thesisTeaching methodscompleted bachelor thesisLanguageGerman-English> Bachelor Exam BAP Molecular Biotechnology moreBachelor Exam BAPLector: FH-Prof. Dr. Herbert Wank0SWS2ECTSLecture contentsThe Bachelor's examination is the final examination of the Bachelor's programme before an examination senate relevant to the subject. The students present results from their work experience in the form of a lecture. Students are interviewed by the examination senate on their presentation as well as on central theoretical and practical topics of the Bachelor's programme.Assessment methodsUp to 20 points are awarded for the presentation by the examination senate. Up to 20 points are also awarded for the subsequent survey for presentation purposes. Up to 30 points are awarded for answering questions on central theoretical and practical topics of the bachelor's programme. The sum of these points results in the overall grade for the Bachelor examination.Teaching methodsActivating methods: presentation and oral examinationLanguageGerman-English> RNA VO Molecular Biotechnology moreRNA VOLector: FH-Prof. Dr. Herbert Wank1SWS2ECTSLecture contentsRNA fundamentals, RNA structure, catalytic RNAs, RNA processing, RNA splicing, RNA editing, riboswitches, RNA applications, non-coding RNAs, RNAi, RNA world, SELEXAssessment methodsFinal exam Written exam at the end of the course.Teaching methodsLectureLanguageEnglish> RNA Analysis Lab VO Molecular Biotechnology moreRNA Analysis Lab VOLector: Kay Holleis, BSc MSc, FH-Prof. Dr. Herbert Wank3SWS3ECTSLecture contentsRNA methods: - Northern blot (glucose/galactose metabolism in yeast, RNA extraction from yeast, denaturing RNA agarose gel, RNA transfer, specific oligonucleotide hybridization, band detection, quantitative PCR) - EMSA (in vitro transcription with T7 RNA polymerase, RNA purification, RNA folding, native polyacrylamide gel electrophoresis, RNA staining using methylene blue, detection of RNP complexes) - RNA stability (temperature dependence, pH dependence, RNAses)Assessment methodsContinuous assessment Continuous assessment - presence, motivation, participation, practical skills (results), written report.Teaching methodsLaboratoryLanguageEnglish> RNA SE Molecular Biotechnology moreRNA SELector: FH-Prof. Dr. Herbert Wank1SWS1ECTSLecture contentsIn this course research papers from internationally respected high-quality journals from the field of RNA research are distributed to the students who read them on their own. The seminar itself then takes the format of a peer-group discussion, where students present the background, the most important findings and the conclusion of the publications. Furthermore, they discuss the strengths and the weaknesses of the publications and make suggestions for improvements.Assessment methodsContinuous assessment Participation in discussionTeaching methodsSeminarLanguageEnglishPublications The publications of Herbert Wank written at FH Campus Wien can be found in our publication database, others in the personal web space.
> Methods of DNA analysis VO Molecular Biotechnology moreMethods of DNA analysis VOLector: FH-Prof. Dr. Herbert Wank1SWS2ECTSLecture contentsBiotechnology recombinant DNA - molecular cloning restriction enzymes, enzymes used for molecular cloning plamids - vectors - cloning systems ligation - transformation expression plasmids - recombinant protein expression molecular cloning strategies bacterial strains for cloning or protein expressionAssessment methodswritten ExamTeaching methodsLectureLanguageGerman
> Molecular Biology & Genetics II VO Molecular Biotechnology moreMolecular Biology & Genetics II VOLector: FH-Prof. Dr. Herbert Wank2SWS3ECTSLecture contentsExpression of the genome • Mechanism of transcription (DNA -> RNA) • Splicing of RNA • Translation (RNA -> protein) • The genetic code Regulation of gene expression • Transcriptional regulation in prokaryotes • Transcriptional regulation in eukaryotes • Regulatory RNAs Gene regulation in development and evolution Basic methods of molecular biology • nucleic acids • proteins Signal transduction cell death model organismsAssessment methodsWritten exam on the last day of the course or in coordination with students.Teaching methodsLecture Powerpoint presentations VideosLanguageGerman-English
> Cell Biology of the Eukaryotes VO Molecular Biotechnology moreCell Biology of the Eukaryotes VOLector: FH-Prof. Dr. Herbert Wank2SWS3ECTSLecture contentsStructure and function of cellular organelles (Nucleus, Mitochondria, Endoplasmatic Reticulum, Golgi, ect.) and substructures (cytoskeleton). Composition, properties and functional relevance of biomembranes. Ion channel and carrier mediated transport of small molecules through membranes. Protein transport into organelles as well as in and out of cells (endocytosis/secretion). The cytoskeleton: composition, regulatory proteins and intracellular transport events. Contact/communication between cells via junctions; the concept of tissues and the extracellular matrix. Complex processes that integrate several features: propagation of action potential along nerve cells; muscle contraction, energy conversion in mitochondriaAssessment methodswritten examTeaching methodsPower point presentationLanguageGerman
> English in Science & Career I ILV Molecular Biotechnology moreEnglish in Science & Career I ILVLector: FH-Prof. Dr. Herbert Wank, FH-Prof. Dr. Paul Watson2SWS2ECTSLecture contentsThe course will deal with all four areas of language skills: reading/writing/talking/listening. The emphasis in the 3rd semester will be on spoken English (presentations (spontaneous and prepared)). Oral presentations will be an important part of the semesters's work. Grammar revision (where appropriate) will be offerred. ACTIVE PARTICIPATION IN THE LESSON IS VERY IMPORTANT AND WILL BE GRADED!Assessment methodsYour command of English, both spoken and written, will be continuously assessed during the semester and will be reflected in the final semester mark. Moreover your active participation in the lessons will also contribute to the overall mark.Teaching methodsSpontaneous and prepared presentations. Brainstormings, Discussions, Debates. ‘Language clinics’. Individual, pair- and group-work. Self-reflection is a major focus of the course.LanguageEnglish
> Industry Practical PR Molecular Biotechnology moreIndustry Practical PRLector: FH-Prof. Dr. Herbert Wank, FH-Prof. Dr. Paul Watson0SWS25ECTSLecture contentsThe internship serves as an introduction for the students to work independently. The tasks begin with the search for a suitable internship and internship supervisor. Students learn under the supervision of a professional the relevant professional practice in a biotechnology company/research institute and/or independent scientific work. During the internship the subject-, methodological and social competencies acquired during the study, will be implemented in the desired field of occupational activity and practically consolidated. Another important teaching content is to summarize the internship results in form of an internship report and the documentation of scientific results.Assessment methodsAssessment of practical supervisorTeaching methodsPracticalLanguageGerman-English
> Molecular Biology & Genetics I VO Molecular Biotechnology moreMolecular Biology & Genetics I VOLector: FH-Prof. Dr. Herbert Wank2SWS3ECTSLecture contentsStudents learn the basics of genetics and molecular biology with the following topics: • Genetics - Mendel • Classic genetics - gene mapping • genetic defects • Nucleic acids (DNA, RNA) - structure and function • Genome structure, chromatin and nucleosomes • chromosomes • Replication of the DNA • cell cycle • Mitosis - meiosis • Mutations and repair mechanisms • Homologous recombination • Sequence-specific recombination • Transposable elementsAssessment methodsWritten exam on the last day of the course or in coordination with students.Teaching methodsLecture Powerpoint presentations VideosLanguageGerman
> Introduction to Molecular Biological Lab Technique… Molecular Biotechnology moreIntroduction to Molecular Biological Lab Techniques LABLector: Kay Holleis, BSc MSc, FH-Prof. Dr. Herbert Wank, FH-Prof. Dr. Paul Watson1SWS1ECTSLecture contentsIn this course the students will get a crash-course in "how to handle an automatic pipet". Then the students perform several restriction digestions in order to map an unknown piece of DNA. At the end of the course, the students determine the concentration of an unknown DNA-Sample spectrophotometrically (including calculation).Assessment methodsEnthusiasm, Protocol in EnglishTeaching methodsIndependent work in the laboratory Introductory remarks from the lector/tutor Independent drawing of a plasmid map Writing of a protocolLanguageGerman
> Genetic Engineering LAB Molecular Biotechnology moreGenetic Engineering LABLector: Kay Holleis, BSc MSc, Anna Koch, BSc, Andrea Krames, BSc MSc, Gregor Sommerkamp, BSc., FH-Prof. Dr. Herbert Wank, ao. Univ.-Prof. Dipl.-Biol. Dr Angela Witte3SWS3ECTSLecture contentsIn this course students learn the basics of cloning. The DNA of an ORF of a phage is amplified using PCR and cloned into the pUC18 vector. Following methods are performed: PCR, restriction digestion, DNA ligation, competent E. coli, plasmid transformation, selection, identification of the clones. Additionally a deletion mutant is analysed by PCR and nucleic acid will be precipitated. The students also write a scientific protocol in "publication-form."Assessment methodsthe grades include: - protocol - lab participation - written testTeaching methodsPractical course with theoretical backgroundLanguageGerman
> Molecular Biological & Biophysical Methods SE Molecular Biotechnology moreMolecular Biological & Biophysical Methods SELector: FH-Prof.in Univ.Doz.in Dr.in Ines Swoboda, FH-Prof. Dr. Herbert Wank1.5SWS3ECTSLecture contentsTopics: Detection of Nucleic Acids PCR Western blot Growth of Microorganisms Antibodies and their use in Molecular Biology Centrifugation Protein Purification Southern and Northern blot Primer and Hybridization Proteinexpression Microarray Flurescence in Molecular Biology Sequencing Isolation and Purification of Nucleic Acids from various Organisms Primer and Hybridisation Primer design for cloning of a geneAssessment methodsPreparation, Oral Presentation, Participation in Discussions, written exam at the end of the seminarTeaching methodsSeminar, topic preparation in small groups (4-5), oral presentation in small groups (9-10), 15 minutes presentation, discussion, preparation of a handoutLanguageGerman
> Gene Expression VO Molecular Biotechnology moreGene Expression VOLector: FH-Prof. Dr. Herbert Wank1SWS2ECTSLecture contentsIn this course, the subject areas of the courses "Molecular Biology and Genetics I and II" from the first year of study are partly repeated, deepened and expanded. The individual needs of the students are addressed, i. H. At the beginning of the course, the subject areas to be dealt with are determined together with the students.Assessment methodswritten examTeaching methodsLectureLanguageGerman
> Protein Expression & Purification LAB Molecular Biotechnology moreProtein Expression & Purification LABLector: Anna Koch, BSc, Andrea Krames, BSc MSc, Gregor Sommerkamp, BSc., FH-Prof. Dr. Herbert Wank, ao. Univ.-Prof. Dipl.-Biol. Dr Angela Witte3SWS3ECTSLecture contentsThe function of genes is central in this internship where students perform a continuous experiment in a bacterial system. The students get to know methods of protein analysis. The expression of a recombinant protein is first studied in small scale (expression cloning in E. coli). The time course of protein expression is analyzed with the aid of Western blots. After upscaling the culture volume under the previously worked out conditions, the recombinant protein is purified by affinity chromatography (HIS-Tag purification) and finally analyzed, dialyzed and the amount of protein obtained is determined quantitatively. Lab Report: in the form of a scientific publication - abstract, introduction, M&M, results, discussion, literature and citations.Assessment methodsFinal grade: lab report, final examination, motivation, cooperation, practical skills (results)Teaching methodsAlternative Methods
> Bachelor Thesis I & Scientific Method SE Molecular Biotechnology moreBachelor Thesis I & Scientific Method SELector: FH-Prof. Dr. Herbert Wank0SWS5ECTSLecture contentsbachelor thesis results frominternshipAssessment methodsGrading of the finished bachelor thesisTeaching methodscompleted bachelor thesisLanguageGerman-English
> Bachelor Exam BAP Molecular Biotechnology moreBachelor Exam BAPLector: FH-Prof. Dr. Herbert Wank0SWS2ECTSLecture contentsThe Bachelor's examination is the final examination of the Bachelor's programme before an examination senate relevant to the subject. The students present results from their work experience in the form of a lecture. Students are interviewed by the examination senate on their presentation as well as on central theoretical and practical topics of the Bachelor's programme.Assessment methodsUp to 20 points are awarded for the presentation by the examination senate. Up to 20 points are also awarded for the subsequent survey for presentation purposes. Up to 30 points are awarded for answering questions on central theoretical and practical topics of the bachelor's programme. The sum of these points results in the overall grade for the Bachelor examination.Teaching methodsActivating methods: presentation and oral examinationLanguageGerman-English
> RNA VO Molecular Biotechnology moreRNA VOLector: FH-Prof. Dr. Herbert Wank1SWS2ECTSLecture contentsRNA fundamentals, RNA structure, catalytic RNAs, RNA processing, RNA splicing, RNA editing, riboswitches, RNA applications, non-coding RNAs, RNAi, RNA world, SELEXAssessment methodsFinal exam Written exam at the end of the course.Teaching methodsLectureLanguageEnglish
> RNA Analysis Lab VO Molecular Biotechnology moreRNA Analysis Lab VOLector: Kay Holleis, BSc MSc, FH-Prof. Dr. Herbert Wank3SWS3ECTSLecture contentsRNA methods: - Northern blot (glucose/galactose metabolism in yeast, RNA extraction from yeast, denaturing RNA agarose gel, RNA transfer, specific oligonucleotide hybridization, band detection, quantitative PCR) - EMSA (in vitro transcription with T7 RNA polymerase, RNA purification, RNA folding, native polyacrylamide gel electrophoresis, RNA staining using methylene blue, detection of RNP complexes) - RNA stability (temperature dependence, pH dependence, RNAses)Assessment methodsContinuous assessment Continuous assessment - presence, motivation, participation, practical skills (results), written report.Teaching methodsLaboratoryLanguageEnglish
> RNA SE Molecular Biotechnology moreRNA SELector: FH-Prof. Dr. Herbert Wank1SWS1ECTSLecture contentsIn this course research papers from internationally respected high-quality journals from the field of RNA research are distributed to the students who read them on their own. The seminar itself then takes the format of a peer-group discussion, where students present the background, the most important findings and the conclusion of the publications. Furthermore, they discuss the strengths and the weaknesses of the publications and make suggestions for improvements.Assessment methodsContinuous assessment Participation in discussionTeaching methodsSeminarLanguageEnglish