Dr.in Elisabeth Riegel Academic Staffelisabeth.riegel@fh-campuswien.ac.at T: +43 1 606 68 77-3543 F: +43 1 606 68 77-3509 Room: OG.F.02 Helmut Qualtinger Gasse 2 1030 WienLectures 2020/21Applied Life Sciences> Toxicology Lab UE Molecular Biotechnology moreToxicology Lab UELector: Dr.in Elisabeth Riegel3SWS3ECTSLecture contentsThe toxicological and therapeutic potential of a small molecule drug will be estimated with a variety of cell based test systems. On the one hand the activating potential of the small molecule on a specific pathway (heat shock response pathway) is analysed and on the other hand possible cytotoxic effects are estimated in a concentration-dependent manner. A broad variety of assays is provided for the students including luciferase reporter assays, Western blot, qPCR, flow cytometry, ELISA and general viability assays. The students themselves select suitable methods.Assessment methodsContinuous assessment Assessment of the work in the laboratory, written exams at the beginning of the laboratory course, discussion of the results after the laboratory course and a written report of the experimentsTeaching methodsPractical course with independent performance of the experiments.LanguageEnglishPublications The publications of Elisabeth Riegel written at FH Campus Wien can be found in our publication database, others in the personal web space.
> Toxicology Lab UE Molecular Biotechnology moreToxicology Lab UELector: Dr.in Elisabeth Riegel3SWS3ECTSLecture contentsThe toxicological and therapeutic potential of a small molecule drug will be estimated with a variety of cell based test systems. On the one hand the activating potential of the small molecule on a specific pathway (heat shock response pathway) is analysed and on the other hand possible cytotoxic effects are estimated in a concentration-dependent manner. A broad variety of assays is provided for the students including luciferase reporter assays, Western blot, qPCR, flow cytometry, ELISA and general viability assays. The students themselves select suitable methods.Assessment methodsContinuous assessment Assessment of the work in the laboratory, written exams at the beginning of the laboratory course, discussion of the results after the laboratory course and a written report of the experimentsTeaching methodsPractical course with independent performance of the experiments.LanguageEnglish