FH-Prof. Dr. Thomas Czerny Academic Staff "Stadt Wien" Endowed Professorship for Cell-Based Test Systems thomas.czerny@fh-campuswien.ac.at T: +43 1 606 68 77-3511 F: +43 1 606 68 77-3509Room: OG.F.06 Helmut Qualtinger Gasse 2 1030 Wien Lectures 2019/20 Applied Life Sciences > Biologicals VOMolecular Biotechnologymore Biologicals VO Lector: FH-Prof. Dr. Thomas Czerny 1 SWS 1 ECTS Lecture contents The lecture “Biologicals” gives an overview over the most important aspects of biotherapeutics (=biologics) which is the fastest growing type of drug gaining more and more importance. Focus of the lecture are the discovery research of therapeutic concepts enabled by engineering of biologics, bioprocess development of biologicals, and manufacturing aspects.Major differences and therapeutic aspects differing between small molecule drugs and biologics are discussed; examples of major classes of biologics will be presented as case studies.Discovery and engineering of therapeutic monoclonal antibodies will be discussed in more detail as this class of therapeutics reflects the biggest class amongst many other biologicals.Approaches and technologies for biopharmaceutical manufacturing and purification will be discussed; major aspects and challenges of protein analytics and physicochemical characterization of biologics will be highlighted. Assessment methods written exam in the last lecture Teaching methods lecture Language English > Biotech Seminar SEMolecular Biotechnologymore Biotech Seminar SE Lector: FH-Prof. Dr. Thomas Czerny, Dr.in Elisabeth Riegel 1 SWS 1 ECTS Lecture contents In this course the students learn strategies of scientific research. In a small group they develop such a strategy, by designing experiments and work packages which they evaluate according to different criteria like costs, effort, efficiency, .. Finally each group presents its strategy. Assessment methods Presentation und discussion of the developed strategy Teaching methods Seminar Language English > Biotech Seminar SEMolecular Biotechnologymore Biotech Seminar SE Lector: FH-Prof. Dr. Thomas Czerny 1 SWS 1 ECTS Lecture contents In this course the students learn strategies of scientific research. In a small group they develop such a strategy, by designing experiments and work packages which they evaluate according to different criteria like costs, effort, efficiency, .. Finally each group presents its strategy. Assessment methods Presentation und discussion of the developed strategy Teaching methods Seminar Language English > Drug Discovery SEMolecular Biotechnologymore Drug Discovery SE Lector: FH-Prof. Dr. Thomas Czerny 1 SWS 1 ECTS Lecture contents In this course research papers from internationally respected high-quality journals from the field of Drug Discovery are distributed to the students who read them on their own. The seminar itself then takes the format of a peer-group discussion, where students present the background, the most important findings and the conclusion of the publications. Furthermore, they discuss the strengths and the weaknesses of the publications and make suggestions for improvements. Assessment methods Final examParticipation in discussion (Seminar paper) Teaching methods Seminar Language English > Drug Target Interactions VOMolecular Biotechnologymore Drug Target Interactions VO Lector: FH-Prof. Dr. Thomas Czerny, Dr. Klaus Rumpel 2 SWS 2 ECTS Lecture contents At the center of this course is the drug and its interaction with target proteins. Methods for determination of protein structures and characterization and quantification of protein ligand interactions are presented. In addition strategies and methods for the optimization of these interactions are discussed (lead optimization, rational design). Furthermore, aspects of pharmacokinetics (ADME, prodrugs) are presented. The underlying principles are demonstrated by selected examples. Assessment methods written exam in the last lecture Teaching methods lecturesPowerpoint presentations and down-loads Language English > Forward and Reverse Genetics LABMolecular Biotechnologymore Forward and Reverse Genetics LAB Lector: FH-Prof. Dr. Thomas Czerny, Dr.in Elisabeth Riegel 3 SWS 3 ECTS Lecture contents Experiments will be performed for genetic manipulation of living organisms and cell culture cells. The program includesBasics of the fish model systemmicroinjection of embryosin situ hybridisation of embryosuse of transgenic lines with inducible expressionmiRNA as a reverse genetics method Assessment methods Assessment of the work in the lab, written exams at the beginning and end of the lab course and a written summary of the experiments. Teaching methods lab work Language English > General Biology VOMolecular Biotechnologymore General Biology VO Lector: FH-Prof. Dr. Thomas Czerny 2 SWS 3 ECTS Lecture contents Darwinian evolution, creationism and intelligent design. Driving forces and mechanisms of evolution, population genetics, phylogeny and formation of species. Physical and chemical basis of life and energy requirements. History of life on our planet and milestones of evolution: origin of life, oxygen, eukaryotes, sexual reproduction and multicellular organisms. Features of pro- and eukrayotic cells and the phylogeny of life. Kingdoms of life: eubacteria, archaea, protists, fungi, plants and animals. Human evolution and the scientific method. Assessment methods a written exam at the end of the lectures Teaching methods Oral presentations > Genome Organization ILVMolecular Biotechnologymore Genome Organization ILV Lector: FH-Prof. Dr. Thomas Czerny 1 SWS 2 ECTS Lecture contents Fundamental principles of signal processing by cells in single and multicellular organisms.Organization of genes and gene regulation.Illustrative discussion of some pathways (e.g. MAP kinase, GPCR, nuclear hormone receptor, NF-kB, Jak/Stat, Wnt, apoptosis and stress pathways).Effects of pathways on gene regulation, cell cycle, cytoskeleton and metabolism.Networking with other pathways - signal networks.Techniques for analyzing signaling pathways.Biological and medical aspects of signaling pathways. Assessment methods Written exam Teaching methods Presentation & Activating Methods > Molecular Genetics VOMolecular Biotechnologymore Molecular Genetics VO Lector: FH-Prof. Dr. Thomas Czerny 1.5 SWS 2 ECTS Lecture contents - Fundamental features of genetics and genetic engineering- Different levels of regulation of gene expression in pro- and eukaryotes- Transcriptional regulation (transcription in eukaryotes, transcriptional activation, properties of transcription factors)- Posttranscriptional regulation (splicing, transport, stability of mRNA, translational control)- Effects of chromatin (composition, histone modifications, regulation, epigenetics)- Examples from signalling pathways Assessment methods Final examWritten exams Teaching methods Lectures with Powerpoint presentations Language English > Regulation of Gene Expression VOMolecular Biotechnologymore Regulation of Gene Expression VO Lector: FH-Prof. Dr. Thomas Czerny 1 SWS 2 ECTS Lecture contents The different levels of regulation of gene expression are discussedTranscriptional regulation (transcription in eukaryotes, transcriptional activation, properties of transcription factors)Posttranscriptional regulation (splicing, transport, stability of mRNA, translational control)Effects of chromatin (composition, histone modifications, regulation, epigenetics)Examples from signalling pathways Assessment methods written exam in the last lecture Teaching methods lecturesPowerpoint presentations and down-loads Language English > Regulation of Gene Expression VOMolecular Biotechnologymore Regulation of Gene Expression VO Lector: FH-Prof. Dr. Thomas Czerny 1 SWS 2 ECTS Lecture contents The different levels of regulation of gene expression are discussedTranscriptional regulation (transcription in eukaryotes, transcriptional activation, properties of transcription factors)Posttranscriptional regulation (splicing, transport, stability of mRNA, translational control)Effects of chromatin (composition, histone modifications, regulation, epigenetics)Examples from signalling pathways Assessment methods written exam in the last lecture Teaching methods lecturesPowerpoint presentations and down-loads Language English > Signalling Pathways VOMolecular Biotechnologymore Signalling Pathways VO Lector: FH-Prof. Dr. Thomas Czerny 1 SWS 2 ECTS Lecture contents Important signalling pathways of the cell (e.g. MAP kinase-, GPCR-, Nuclear Hormone Receptor-, NF-kB-, Jak/Stat-, Wnt-, Hedgehog-, Tgfß-, Apoptosis-, PI3K/Akt- and stress pathways) are presented together with their effects on gene expression and other functions of the cell. In addition the crosslinks with other pathways are discussed. Techniques for the analysis of signalling pathways are also presented. Assessment methods Final examWritten Exam Teaching methods Lectures with Powerpoint presentations, Discussion of selected chapters Language English PublicationsThe publications of Thomas Czerny written at FH Campus Wien can be found in our publication database, others in the personal web space.
> Biologicals VOMolecular Biotechnologymore Biologicals VO Lector: FH-Prof. Dr. Thomas Czerny 1 SWS 1 ECTS Lecture contents The lecture “Biologicals” gives an overview over the most important aspects of biotherapeutics (=biologics) which is the fastest growing type of drug gaining more and more importance. Focus of the lecture are the discovery research of therapeutic concepts enabled by engineering of biologics, bioprocess development of biologicals, and manufacturing aspects.Major differences and therapeutic aspects differing between small molecule drugs and biologics are discussed; examples of major classes of biologics will be presented as case studies.Discovery and engineering of therapeutic monoclonal antibodies will be discussed in more detail as this class of therapeutics reflects the biggest class amongst many other biologicals.Approaches and technologies for biopharmaceutical manufacturing and purification will be discussed; major aspects and challenges of protein analytics and physicochemical characterization of biologics will be highlighted. Assessment methods written exam in the last lecture Teaching methods lecture Language English
> Biotech Seminar SEMolecular Biotechnologymore Biotech Seminar SE Lector: FH-Prof. Dr. Thomas Czerny, Dr.in Elisabeth Riegel 1 SWS 1 ECTS Lecture contents In this course the students learn strategies of scientific research. In a small group they develop such a strategy, by designing experiments and work packages which they evaluate according to different criteria like costs, effort, efficiency, .. Finally each group presents its strategy. Assessment methods Presentation und discussion of the developed strategy Teaching methods Seminar Language English
> Biotech Seminar SEMolecular Biotechnologymore Biotech Seminar SE Lector: FH-Prof. Dr. Thomas Czerny 1 SWS 1 ECTS Lecture contents In this course the students learn strategies of scientific research. In a small group they develop such a strategy, by designing experiments and work packages which they evaluate according to different criteria like costs, effort, efficiency, .. Finally each group presents its strategy. Assessment methods Presentation und discussion of the developed strategy Teaching methods Seminar Language English
> Drug Discovery SEMolecular Biotechnologymore Drug Discovery SE Lector: FH-Prof. Dr. Thomas Czerny 1 SWS 1 ECTS Lecture contents In this course research papers from internationally respected high-quality journals from the field of Drug Discovery are distributed to the students who read them on their own. The seminar itself then takes the format of a peer-group discussion, where students present the background, the most important findings and the conclusion of the publications. Furthermore, they discuss the strengths and the weaknesses of the publications and make suggestions for improvements. Assessment methods Final examParticipation in discussion (Seminar paper) Teaching methods Seminar Language English
> Drug Target Interactions VOMolecular Biotechnologymore Drug Target Interactions VO Lector: FH-Prof. Dr. Thomas Czerny, Dr. Klaus Rumpel 2 SWS 2 ECTS Lecture contents At the center of this course is the drug and its interaction with target proteins. Methods for determination of protein structures and characterization and quantification of protein ligand interactions are presented. In addition strategies and methods for the optimization of these interactions are discussed (lead optimization, rational design). Furthermore, aspects of pharmacokinetics (ADME, prodrugs) are presented. The underlying principles are demonstrated by selected examples. Assessment methods written exam in the last lecture Teaching methods lecturesPowerpoint presentations and down-loads Language English
> Forward and Reverse Genetics LABMolecular Biotechnologymore Forward and Reverse Genetics LAB Lector: FH-Prof. Dr. Thomas Czerny, Dr.in Elisabeth Riegel 3 SWS 3 ECTS Lecture contents Experiments will be performed for genetic manipulation of living organisms and cell culture cells. The program includesBasics of the fish model systemmicroinjection of embryosin situ hybridisation of embryosuse of transgenic lines with inducible expressionmiRNA as a reverse genetics method Assessment methods Assessment of the work in the lab, written exams at the beginning and end of the lab course and a written summary of the experiments. Teaching methods lab work Language English
> General Biology VOMolecular Biotechnologymore General Biology VO Lector: FH-Prof. Dr. Thomas Czerny 2 SWS 3 ECTS Lecture contents Darwinian evolution, creationism and intelligent design. Driving forces and mechanisms of evolution, population genetics, phylogeny and formation of species. Physical and chemical basis of life and energy requirements. History of life on our planet and milestones of evolution: origin of life, oxygen, eukaryotes, sexual reproduction and multicellular organisms. Features of pro- and eukrayotic cells and the phylogeny of life. Kingdoms of life: eubacteria, archaea, protists, fungi, plants and animals. Human evolution and the scientific method. Assessment methods a written exam at the end of the lectures Teaching methods Oral presentations
> Genome Organization ILVMolecular Biotechnologymore Genome Organization ILV Lector: FH-Prof. Dr. Thomas Czerny 1 SWS 2 ECTS Lecture contents Fundamental principles of signal processing by cells in single and multicellular organisms.Organization of genes and gene regulation.Illustrative discussion of some pathways (e.g. MAP kinase, GPCR, nuclear hormone receptor, NF-kB, Jak/Stat, Wnt, apoptosis and stress pathways).Effects of pathways on gene regulation, cell cycle, cytoskeleton and metabolism.Networking with other pathways - signal networks.Techniques for analyzing signaling pathways.Biological and medical aspects of signaling pathways. Assessment methods Written exam Teaching methods Presentation & Activating Methods
> Molecular Genetics VOMolecular Biotechnologymore Molecular Genetics VO Lector: FH-Prof. Dr. Thomas Czerny 1.5 SWS 2 ECTS Lecture contents - Fundamental features of genetics and genetic engineering- Different levels of regulation of gene expression in pro- and eukaryotes- Transcriptional regulation (transcription in eukaryotes, transcriptional activation, properties of transcription factors)- Posttranscriptional regulation (splicing, transport, stability of mRNA, translational control)- Effects of chromatin (composition, histone modifications, regulation, epigenetics)- Examples from signalling pathways Assessment methods Final examWritten exams Teaching methods Lectures with Powerpoint presentations Language English
> Regulation of Gene Expression VOMolecular Biotechnologymore Regulation of Gene Expression VO Lector: FH-Prof. Dr. Thomas Czerny 1 SWS 2 ECTS Lecture contents The different levels of regulation of gene expression are discussedTranscriptional regulation (transcription in eukaryotes, transcriptional activation, properties of transcription factors)Posttranscriptional regulation (splicing, transport, stability of mRNA, translational control)Effects of chromatin (composition, histone modifications, regulation, epigenetics)Examples from signalling pathways Assessment methods written exam in the last lecture Teaching methods lecturesPowerpoint presentations and down-loads Language English
> Regulation of Gene Expression VOMolecular Biotechnologymore Regulation of Gene Expression VO Lector: FH-Prof. Dr. Thomas Czerny 1 SWS 2 ECTS Lecture contents The different levels of regulation of gene expression are discussedTranscriptional regulation (transcription in eukaryotes, transcriptional activation, properties of transcription factors)Posttranscriptional regulation (splicing, transport, stability of mRNA, translational control)Effects of chromatin (composition, histone modifications, regulation, epigenetics)Examples from signalling pathways Assessment methods written exam in the last lecture Teaching methods lecturesPowerpoint presentations and down-loads Language English
> Signalling Pathways VOMolecular Biotechnologymore Signalling Pathways VO Lector: FH-Prof. Dr. Thomas Czerny 1 SWS 2 ECTS Lecture contents Important signalling pathways of the cell (e.g. MAP kinase-, GPCR-, Nuclear Hormone Receptor-, NF-kB-, Jak/Stat-, Wnt-, Hedgehog-, Tgfß-, Apoptosis-, PI3K/Akt- and stress pathways) are presented together with their effects on gene expression and other functions of the cell. In addition the crosslinks with other pathways are discussed. Techniques for the analysis of signalling pathways are also presented. Assessment methods Final examWritten Exam Teaching methods Lectures with Powerpoint presentations, Discussion of selected chapters Language English