Personendetail

Katharina Seiberl, BSc MSc

Research Staff


T: +43 1 606 68 77-3611
F: +43 1 606 68 77-3609

Room: MUT.03
Muthgasse 11/2
1190 Wien


Lectures 2021/22

Applied Life Sciences

> Biochemistry (practical Course) LB
Bioengineering more

Biochemistry (practical Course) LB

Lector: Simon Gerner, BSc, Ing. DI (FH) Dr. Harald Kühnel, MSc, Katharina Seiberl, BSc MSc, Katharina Stadlbauer, BSc, DI Dr. Gerhard Stadlmayr

2.5SWS
3ECTS

Lecture contents

Chemical Calculations, NiNTA Chromatopraphy, Dialysis, Enzyme activity assays, Enzyme kinetic assays, SEC and HPLC, Protein quantification (Bradford, OD280), SDS-PAGE, Western Blot, ELISA

Assessment methods

In-class participation and written protocols

Teaching methods

Practical training accompanied by discussion and reflection rounds

Language

German

> Molecular Genetics (practical Course) LB
Bioengineering more

Molecular Genetics (practical Course) LB

Lector: Ing. DI (FH) Dr. Harald Kühnel, MSc, Dr. Christian Leitner, Katharina Seiberl, BSc MSc

2SWS
3ECTS

Lecture contents

Construction of an expression vector to produce l-lactic acid in Saccharomyces cerevisiae
1.) Isolation of the l-lactat-dehydrogenase gen form Lactobacillus plantarum
2.) Construction and amplification of the expression vector in E. coli
3.) Transferring the expression vector in the target organism S. cerevisiae and measurement of the LDH activity

Assessment methods

Writing a scientific lab protocol
Recommended language: German or English
Rated will be the protocol and the practical work in the lab.

Teaching methods

After a theoretical introduction at the beginning of each lab day the students will conduct the experiments by themselves with help from the protocols.

Language

German

> Practical Course: Fermentation Technology LB
Bioengineering more

Practical Course: Fermentation Technology LB

Lector: Ing. Michael Geissler, MSc., Mathias Gyenge, BSc, Ing. DI (FH) Dr. Harald Kühnel, MSc, Dr. Christian Leitner, DI Dr. Nico Lingg, FH-Prof. DI Dr. Michael Maurer, Katharina Seiberl, BSc MSc

3.5SWS
5ECTS

Lecture contents

The course consists of two parts:
1. Brewing technology
2. Characterization of a recombinant yeast strain
In the course of the brewing technology, beer, liquor and vinegar are produced. This includes the process design, QA, filling and labeling.
In the second part, a yeast strain producing lactic acid (outcome of the molecular biological lab) will be characterized in terms of productivity in comparison to the wild type and a mutant strain (PDC neg.)

Assessment methods

Written reports

Teaching methods

Laboratory

Language

German

> Fermentation (Practical Course) LB
Bioengineering more

Fermentation (Practical Course) LB

Lector: Mag. Dipl.-Ing. Dr. Martin Pfeffer, Mag. Karin Pfeffer, Katharina Seiberl, BSc MSc

1.5SWS
2ECTS

Lecture contents

The aim of this course is the design, operation and analysis of a controlled bioprocess. Therefore, the recombinant E. coli strain will be cultivated in a batch fermentation.

Assessment methods

- colloquium

- report

- active participation

Teaching methods

group work

Language

German

> Measurement, Control and Automation ILV
Bioprocess Technology more

Measurement, Control and Automation ILV

Lector: Prof. Dr.-Ing. Richard Biener, Katharina Seiberl, BSc MSc

1.5SWS
3ECTS

Lecture contents

introduction to control theory
Controllers (PID-control, two-position control, cascade control,…)
requirement to control loops (performance, stability)
control design, determination of controller paameters
control of bioreactors (temperature, pH, pO2)
practical design of flow and level control

Assessment methods

written exam

Teaching methods

lecture
exercises
practical training

Language

German

> Plant Cleaning ILV
Bioprocess Technology more

Plant Cleaning ILV

Lector: DI (FH) Sandra Leupold, DI Dr. Karl Metzger, Katharina Seiberl, BSc MSc

1SWS
2ECTS

Lecture contents

Cleaning of surfaces, that are touched by products
Matrices of processes and tools
Analysis and planning of cleaning validation
Exercise example for cleaning and cleaning validation at a fermenter or a similar processing unit

Assessment methods

Continous assessments throughout the course

Teaching methods

After an introductory course concerning theory and experience of cleaning, as well as the regulatory framework, an exercise course with a fermentation equipment is following.

Language

German-English

> Downstream Processing (Practical Course) LB
Bioprocess Technology more

Downstream Processing (Practical Course) LB

Lector: Dipl.-Ing. Anna-Carina Frank, DI Dr. Nico Lingg, Markus Mozgovicz, BSc., Dr. Monika Müller, Dipl.-Ing. Dr. Julita Panek, Katharina Seiberl, BSc MSc

3SWS
6ECTS

Lecture contents

General aspects of Downstreamprocessing in the environement of a biotechnology production Unit operations of DSP (Centrifugation, precipitation, filtration, ultrafiltration, chromatographic methods). Dimensional analysis of unit operation with simple models.

Assessment methods

Written examination

Teaching methods

Lecture with sample calculation

Language

German

> Bioprocess Engineering (Practical Course) LB
Bioprocess Technology more

Bioprocess Engineering (Practical Course) LB

Lector: Atefeh Ebrahimian, DI Dr. Karl Metzger, Dipl.-Ing. Harald Schillinger, Katharina Seiberl, BSc MSc, DI (FH) Florian Strobl, MSc

2SWS
4ECTS

Lecture contents

The bioprocess laboratory involves the production process. It consists of designing the process to harvesting and filtration.
The students will learn the production of the “Green Fluorescent Protein” with E. coli.

Assessment methods

report and presentation

Teaching methods

Practical course

Language

German-English

> Data Analysis Laboratory LB
Bioinformatics more

Data Analysis Laboratory LB

Lector: Ing. DI (FH) Dr. Harald Kühnel, MSc, Katharina Seiberl, BSc MSc, Monika Waldherr, MSc

2SWS
4ECTS

Lecture contents

-) Introduction to the Oxford Nanopore sequencing technology
-) Sequencing of DNA samples using MinION in the wet lab
-) Development of suitable quality control
-) Development of suitable analysis pipeline
-) Interpretation and discussion of results

Assessment methods

Immanent examination, protocol of the results of the practical exercises, final exam

Teaching methods

-) Presentation
-) practical exercises in the wet-lab and in-silico

Language

German

> Downstream Processing (Practical Course) LB
Bioengineering more

Downstream Processing (Practical Course) LB

Lector: Dipl.-Ing. Anna-Carina Frank, Viktoria Mayer, MSc, Katharina Seiberl, BSc MSc

1SWS
1ECTS

Lecture contents

The recombinant proteins produced in the fermentation laboratory course will be purified with typical unit operation. The course covers homogenization, centrifugation, chromatography and ultra-/diafiltration.

Assessment methods

- Colloquium

- Protocol

- Participation

Teaching methods

Pactical course in groups

> Wastewater Treatment ILV
Bioprocess Technology more

Wastewater Treatment ILV

Lector: Mag. Dr. Andreas Franz, Katharina Seiberl, BSc MSc

1.5SWS
3ECTS

Lecture contents

This course is held in German. The description is therefore also restricted to German.

Assessment methods

This course is held in German. The description is therefore also restricted to German.

Teaching methods

This course is held in German. The description is therefore also restricted to German.

Language

German

> Fermentation Practical LB
Bioprocess Technology more

Fermentation Practical LB

Lector: Ing. Michael Geissler, MSc., FH-Prof. DI Dr. Michael Maurer, Katharina Seiberl, BSc MSc

1SWS
2ECTS

Lecture contents

Brewing at a Craft Beer Brewery:
> Recipe design
> Scale up 50L -> 10hL and comparison
> QA/QC of brewing process
> Filling - KEGs and bottles
> Marketing

Assessment methods

Protocol

Teaching methods

practical course

> Molecular Biology Laboratory Practical LB
Bioprocess Technology more

Molecular Biology Laboratory Practical LB

Lector: Atefeh Ebrahimian, Ing. DI (FH) Dr. Harald Kühnel, MSc, Katharina Seiberl, BSc MSc

1SWS
2ECTS

Lecture contents

Introduction to eukaryotic (mammalian) cell culture
Basic Methods: Thawing, Splitting, Seeding, Harvesting, Freezing,
Transfection methods: Co-precipitation and lipofection
Influence of e.g. different plasmid backbones or different host cell lines on product
Analysis: Fluorometric, cytotoxicity, protein content (specific vs. total)

Assessment methods

Continuous assessment of course work/protocol

Teaching methods

Lecture, experimentation, discussion

Language

German-English