Dr. Elisabeth Riegel Academic Staffelisabeth.riegel@fh-campuswien.ac.at T: +43 1 606 68 77-3543 F: +43 1 606 68 77-3509 Room: OG.F.02 Helmut Qualtinger Gasse 2 1030 WienLectures 2021/22Applied Life Sciences> Signalling Pathways Lab UE Molecular Biotechnology moreSignalling Pathways Lab UELector: Dr.in Elisabeth Riegel3SWS3ECTSLecture contentsMethods for the manipulation and the analysis of signalling pathways in cell culture are applied, broadening the knowledge on specific pathways. The applied methods are transient transfection in cell culture, reporter constructs with gfp and luciferase, overexpression of activators/repressors (including RNAi), Western analysis of cellular extracts, analysis of phosphorylation, fluorescence microscopy of labelled proteins and pharmacologic manipulation of the pathways.Assessment methodsFinal exam Assessment of the work in the laboratory, written exams at the beginning of the laboratory course, discussion of the results after the laboratory course and a written report of the experiments. (Seminar paper)Teaching methodsLaboratory workLanguageEnglish> Toxicology Lab UE Molecular Biotechnology moreToxicology Lab UELector: Kay Holleis, BSc MSc, Dr.in Elisabeth Riegel3SWS3ECTSLecture contentsThe toxicological and therapeutic potential of a small molecule drug will be estimated with a variety of cell based test systems. On the one hand the activating potential of the small molecule on a specific pathway (heat shock response pathway) is analysed and on the other hand possible cytotoxic effects are estimated in a concentration-dependent manner. A broad variety of assays is provided for the students including luciferase reporter assays, Western blot, qPCR, flow cytometry, ELISA and general viability assays. The students themselves select suitable methods.Assessment methodsContinuous assessment Assessment of the work in the laboratory, written exams at the beginning of the laboratory course, discussion of the results after the laboratory course and a written report of the experimentsTeaching methodsPractical course with independent performance of the experiments.LanguageEnglish> Drug Discovery SE Molecular Biotechnology moreDrug Discovery SELector: FH-Prof. Dr. Thomas Czerny, Dr.in Elisabeth Riegel1SWS1ECTSLecture contentsIn this course research papers from internationally respected high-quality journals from the field of Drug Discovery are distributed to the students who read them on their own. The seminar itself then takes the format of a peer-group discussion, where students present the background, the most important findings and the conclusion of the publications. Furthermore, they discuss the strengths and the weaknesses of the publications and make suggestions for improvements.Assessment methodsFinal exam Participation in discussion (Seminar paper)Teaching methodsSeminarLanguageEnglish> Scientific Method: Drug Discovery SE Molecular Biotechnology moreScientific Method: Drug Discovery SELector: FH-Prof. Dr. Thomas Czerny, Dr.in Elisabeth Riegel1SWS1ECTSLecture contentsIn this course the students learn strategies of scientific research. In a small group they develop a strategy for a given topic (in Drug Discovery), by designing experiments and work packages which they evaluate according to different criteria like costs, effort, efficiency, Finally each group presents its research project.Assessment methodsFinal exam Presentation and discussion of the developed strategyTeaching methodsSeminarLanguageEnglishPublications The publications of Elisabeth Riegel written at FH Campus Wien can be found in our publication database, others in the personal web space.
> Signalling Pathways Lab UE Molecular Biotechnology moreSignalling Pathways Lab UELector: Dr.in Elisabeth Riegel3SWS3ECTSLecture contentsMethods for the manipulation and the analysis of signalling pathways in cell culture are applied, broadening the knowledge on specific pathways. The applied methods are transient transfection in cell culture, reporter constructs with gfp and luciferase, overexpression of activators/repressors (including RNAi), Western analysis of cellular extracts, analysis of phosphorylation, fluorescence microscopy of labelled proteins and pharmacologic manipulation of the pathways.Assessment methodsFinal exam Assessment of the work in the laboratory, written exams at the beginning of the laboratory course, discussion of the results after the laboratory course and a written report of the experiments. (Seminar paper)Teaching methodsLaboratory workLanguageEnglish
> Toxicology Lab UE Molecular Biotechnology moreToxicology Lab UELector: Kay Holleis, BSc MSc, Dr.in Elisabeth Riegel3SWS3ECTSLecture contentsThe toxicological and therapeutic potential of a small molecule drug will be estimated with a variety of cell based test systems. On the one hand the activating potential of the small molecule on a specific pathway (heat shock response pathway) is analysed and on the other hand possible cytotoxic effects are estimated in a concentration-dependent manner. A broad variety of assays is provided for the students including luciferase reporter assays, Western blot, qPCR, flow cytometry, ELISA and general viability assays. The students themselves select suitable methods.Assessment methodsContinuous assessment Assessment of the work in the laboratory, written exams at the beginning of the laboratory course, discussion of the results after the laboratory course and a written report of the experimentsTeaching methodsPractical course with independent performance of the experiments.LanguageEnglish
> Drug Discovery SE Molecular Biotechnology moreDrug Discovery SELector: FH-Prof. Dr. Thomas Czerny, Dr.in Elisabeth Riegel1SWS1ECTSLecture contentsIn this course research papers from internationally respected high-quality journals from the field of Drug Discovery are distributed to the students who read them on their own. The seminar itself then takes the format of a peer-group discussion, where students present the background, the most important findings and the conclusion of the publications. Furthermore, they discuss the strengths and the weaknesses of the publications and make suggestions for improvements.Assessment methodsFinal exam Participation in discussion (Seminar paper)Teaching methodsSeminarLanguageEnglish
> Scientific Method: Drug Discovery SE Molecular Biotechnology moreScientific Method: Drug Discovery SELector: FH-Prof. Dr. Thomas Czerny, Dr.in Elisabeth Riegel1SWS1ECTSLecture contentsIn this course the students learn strategies of scientific research. In a small group they develop a strategy for a given topic (in Drug Discovery), by designing experiments and work packages which they evaluate according to different criteria like costs, effort, efficiency, Finally each group presents its research project.Assessment methodsFinal exam Presentation and discussion of the developed strategyTeaching methodsSeminarLanguageEnglish