Dr.in Elisabeth Riegel Academic Staff elisabeth.riegel@fh-campuswien.ac.at T: +43 1 606 68 77-3543 F: +43 1 606 68 77-3509Room: OG.F.02 Helmut Qualtinger Gasse 2 1030 Wien Lectures 2019/20 Applied Life Sciences > Biotech Seminar SEMolecular Biotechnologymore Biotech Seminar SE Lector: FH-Prof. Dr. Thomas Czerny, Dr.in Elisabeth Riegel 1 SWS 1 ECTS Lecture contents In this course the students learn strategies of scientific research. In a small group they develop such a strategy, by designing experiments and work packages which they evaluate according to different criteria like costs, effort, efficiency, .. Finally each group presents its strategy. Assessment methods Presentation und discussion of the developed strategy Teaching methods Seminar Language English > Forward and Reverse Genetics LABMolecular Biotechnologymore Forward and Reverse Genetics LAB Lector: Dr.in Elisabeth Riegel, Andrea Steinbauer, BSc MSc 3 SWS 3 ECTS Lecture contents Experiments will be performed for genetic manipulation of living organisms and cell culture cells. The program includesBasics of the fish model systemmicroinjection of embryosin situ hybridisation of embryosuse of transgenic lines with inducible expressionmiRNA as a reverse genetics method Assessment methods Assessment of the work in the lab, written exams at the beginning and end of the lab course and a written summary of the experiments. Teaching methods lab work Language English > Forward and Reverse Genetics LABMolecular Biotechnologymore Forward and Reverse Genetics LAB Lector: FH-Prof. Dr. Thomas Czerny, Dr.in Elisabeth Riegel 3 SWS 3 ECTS Lecture contents Experiments will be performed for genetic manipulation of living organisms and cell culture cells. The program includesBasics of the fish model systemmicroinjection of embryosin situ hybridisation of embryosuse of transgenic lines with inducible expressionmiRNA as a reverse genetics method Assessment methods Assessment of the work in the lab, written exams at the beginning and end of the lab course and a written summary of the experiments. Teaching methods lab work Language English > Signalling Pathways Lab UEMolecular Biotechnologymore Signalling Pathways Lab UE Lector: Dr.in Elisabeth Riegel, Andrea Steinbauer, BSc MSc 3 SWS 3 ECTS Lecture contents Methods for the manipulation and the analysis of signalling pathways in cell culture are applied, broadening the knowledge on specific pathways. The applied methods are transient transfection in cell culture, reporter constructs with gfp and luciferase, overexpression of activators/repressors (including RNAi), Western analysis of cellular extracts, analysis of phosphorylation, fluorescence microscopy of labelled proteins and pharmacologic manipulation of the pathways. Assessment methods Final examAssessment of the work in the laboratory, written exams at the beginning of the laboratory course, discussion of the results after the laboratory course and a written report of the experiments. (Seminar paper) Teaching methods Laboratory work Language English PublicationsThe publications of Elisabeth Riegel written at FH Campus Wien can be found in our publication database, others in the personal web space.
> Biotech Seminar SEMolecular Biotechnologymore Biotech Seminar SE Lector: FH-Prof. Dr. Thomas Czerny, Dr.in Elisabeth Riegel 1 SWS 1 ECTS Lecture contents In this course the students learn strategies of scientific research. In a small group they develop such a strategy, by designing experiments and work packages which they evaluate according to different criteria like costs, effort, efficiency, .. Finally each group presents its strategy. Assessment methods Presentation und discussion of the developed strategy Teaching methods Seminar Language English
> Forward and Reverse Genetics LABMolecular Biotechnologymore Forward and Reverse Genetics LAB Lector: Dr.in Elisabeth Riegel, Andrea Steinbauer, BSc MSc 3 SWS 3 ECTS Lecture contents Experiments will be performed for genetic manipulation of living organisms and cell culture cells. The program includesBasics of the fish model systemmicroinjection of embryosin situ hybridisation of embryosuse of transgenic lines with inducible expressionmiRNA as a reverse genetics method Assessment methods Assessment of the work in the lab, written exams at the beginning and end of the lab course and a written summary of the experiments. Teaching methods lab work Language English
> Forward and Reverse Genetics LABMolecular Biotechnologymore Forward and Reverse Genetics LAB Lector: FH-Prof. Dr. Thomas Czerny, Dr.in Elisabeth Riegel 3 SWS 3 ECTS Lecture contents Experiments will be performed for genetic manipulation of living organisms and cell culture cells. The program includesBasics of the fish model systemmicroinjection of embryosin situ hybridisation of embryosuse of transgenic lines with inducible expressionmiRNA as a reverse genetics method Assessment methods Assessment of the work in the lab, written exams at the beginning and end of the lab course and a written summary of the experiments. Teaching methods lab work Language English
> Signalling Pathways Lab UEMolecular Biotechnologymore Signalling Pathways Lab UE Lector: Dr.in Elisabeth Riegel, Andrea Steinbauer, BSc MSc 3 SWS 3 ECTS Lecture contents Methods for the manipulation and the analysis of signalling pathways in cell culture are applied, broadening the knowledge on specific pathways. The applied methods are transient transfection in cell culture, reporter constructs with gfp and luciferase, overexpression of activators/repressors (including RNAi), Western analysis of cellular extracts, analysis of phosphorylation, fluorescence microscopy of labelled proteins and pharmacologic manipulation of the pathways. Assessment methods Final examAssessment of the work in the laboratory, written exams at the beginning of the laboratory course, discussion of the results after the laboratory course and a written report of the experiments. (Seminar paper) Teaching methods Laboratory work Language English